Pathophysiological significance of a reaction in mouse gastrointestinal tract associated with delayed-type hypersensitivity

Figure 1 Effect of DNCB gavage on gastrointestinal motility in sensitized mice. ^bP < 0.01 vs control, ^cP < 0.05 vs DTH_(-), ^dP < 0.01 vs DTH_(-) and DTH₁n = 8 in control; n = 7 in DTH_(-), DTH₁ and DTH₂ groups.

Figure 2 Effect of DNCB enema on DAI score in sensitized mice. ^aP < 0.05 vs control. n = 16 in control group and DTH₄ group; n = 11 in DTH₅ group; n = 17 in DTH₆ group.

Figure 3 The pathological change of colon by DNCB enema in sensitized mice. A1: control 600 mL/L ethanol, normal histological structures. A2: DTH_(-), only slight mucosa congestion. B: 1 g/L DNCB, hyperemia, infiltration of chronic inflammatory. cells, decrease of the number of goblet cell in colon mucosa. C: 2 g/L DNCB, superficial erosion, chronic inflammation of colon mucosa. D: 4 g/L DNCB, atrophic changes of colon mucosa with chronic inflammation. E: 4 g/L DNCB, ulcer and hemorrhage in colon mucosa (HE, ×200).

Figure 4 Pathological score in the mice received DNCB enema after sensitization. 4A: macropathology; 4B: microscopically observation. group 1: control (600 mL/L ethanol, n = 16); group 2: DTH₄ (1 g/L DNCB, n = 17); group 3: DTH ₅ (2 g/L DNCB, n = 17); and group 4: DTH₆ (4 g/L DNCB, n = 17) ^aP < 0.05 vs group 4A1 and 4B1, respectively.