Cloning and expression of mouse peroxiredoxin I in IEC-6 Cells

Figure 1 Amplification of Peroxiredoxin I by RT-PCR. A: total RNAs were isolated from mouse intestinal epithelia; three bands were visualized on agarose gel, which represented the main ribosome RNA of the cells. B: amplification of the coding region of peroxiredoxin I applying RT-PCR.

Figure 2 Recombinant expression vector pSG5-Prx. A: diagrams of the expression vector pSG5-Prx. B: electrophoresis map of this vector (EcoR I and BamH I). λ/HindIII marker was loaded to estimate the relative molecular weight of the expression vector.

Figure 3 Detection of the transcription of recombinant vector pSG5-Prx by RT-PCR. Total RNAs isolated from transfected cells were applying for RT-PCR analysis with primers P2 and P3. Lane 1: DNA marker DL 2000, Lane 2: RT-PCR product, Lane 3: PCR product using purified recombinant vector as template.

Figure 4 Immunoblotting of peroxiredoxin I of transfected and normal IEC-6 cell.