Loss of heterozygosity on chromosome 10q22-10q23 and 22q11.2-22q12.1 and p53 gene in primary hepatocellular carcinoma

Figure 1 Agarose gel electrophoresis of PCR products of p53 gene exons 2-3, 4, 11, and chromosome 10q22-10q23 and 22q11. 2-22q12. 1. A: PCR products of p53 exon 2-3. Lane 1, 100 bp DNA marker; Lanes 2-8, PCR products of p53 exon2-3 (A) and CDK4 gene (B, as a control) amplified from HCC genomic DNA. No homozygous deletion of p53 exon 2-3 was found in all HCC specimens. B: PCR products of p53 exon 4. Lane 1, 100 bp DNA marker; Lanes 2-8, PCR products of p53 exon4 (A) and CDK4 gene (B, as a control) amplified from HCC genomic DNA. Lines 4, 6-8, homozygous deletion. C: PCR products of p53 exon11. Line 1, 100 bp DNA ladder; Lanes 2-8, PCR products of p53 exon11 (A) and CDK4 gene (B, as a control) amplified from HCC genomic DNA. Lanes 5 and 7, homozygous deletion. D: PCR products of 10q22-10q23. Lane1, 100 bp DNA marker; Lanes 2-8, PCR products of 10q22-10q23 (A) and CDK4 gene (B, as a control) amplified from HCC genomic DNA. Lines 6 and 7, homozygous deletion. E: PCR products of 22q11.2-22q12.1. Lane 1, 100 bp DNA ladder; Lanes 2-8, PCR products of 22q11.2-22q12.1 (A) and CDK4 gene (B, as a control) amplified from HCC genomic DNA. Lanes 7 and 8, homozygous deletion.

Figure 2 Representative illustrations of LOH detected with the microsatellite markers TP53. A, TP53.B, TP53.G, D10S579, D22S421 in human hepatocellular carcinomas (C) as compared to non-tumor liver tissues (N). The arrows show the location of the missing alleles. A: TP53.A (case 4); B: TP53.B (case 17); C: TP53.G (case 19,no LOH); D: D10S579 (case 17); E: D22S421 (case 20).