Zhu GN, Zuo L, Zhou Q, Zhang SM, Zhu HQ, Gui SY, Wang Y. Loss of heterozygosity on chromosome 10q22-10q23 and 22q11.2-22q12.1 and p53 gene in primary hepatocellular carcinoma. World J Gastroenterol 2004; 10(13): 1975-1978 [PMID: 15222050 DOI: 10.3748/wjg.v10.i13.1975]
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Professor Yuan Wang, Laboratory of Molecular Biology and Department of Biochemistry, Anhui Medical University, Hefei 230032, Anhui Province, China. wangyuan@mail.hf.ah.cn
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Zhu GN, Zuo L, Zhou Q, Zhang SM, Zhu HQ, Gui SY, Wang Y. Loss of heterozygosity on chromosome 10q22-10q23 and 22q11.2-22q12.1 and p53 gene in primary hepatocellular carcinoma. World J Gastroenterol 2004; 10(13): 1975-1978 [PMID: 15222050 DOI: 10.3748/wjg.v10.i13.1975]
World J Gastroenterol. Jul 1, 2004; 10(13): 1975-1978 Published online Jul 1, 2004. doi: 10.3748/wjg.v10.i13.1975
Loss of heterozygosity on chromosome 10q22-10q23 and 22q11.2-22q12.1 and p53 gene in primary hepatocellular carcinoma
Guang-Neng Zhu, Li Zuo, Qing Zhou, Su-Mei Zhang, Hua-Qing Zhu, Shu-Yu Gui, Yuan Wang
Guang-Neng Zhu, Li Zuo, Qing Zhou, Su-Mei Zhang, Hua-Qing Zhu, Shu-Yu Gui, Yuan Wang, Laboratory of molecular Biology and Department of Biochemistry, Anhui Medical University, Hefei 230032, Anhui Province, China
Li Zuo, Qing Zhou, Su-Mei Zhang, Hua-Qing Zhu, Shu-Yu Gui, Yuan Wang, Anhui Province Key Laboratory of Genomic Research, Hefei 230032, Anhui Province, China
Guang-Neng Zhu, Department of Biochemistry, Bengbu Medical College, Bengbu 233003, Anhui Province, China
Shu-Yu Gui, Department of Respiratory Disease, the First Affiliated Hospital of Anhui Medical University, Hefei 230032, Anhui Province, China
Author contributions: All authors contributed equally to the work.
Supported by the Natural Science Foundation of Anhui Province, No.99044312 (YW), No.01043716 (SYG) and Natural Science Foundation of Anhui Educational Commission, No.JL-97-077 (YW)
Correspondence to: Professor Yuan Wang, Laboratory of Molecular Biology and Department of Biochemistry, Anhui Medical University, Hefei 230032, Anhui Province, China. wangyuan@mail.hf.ah.cn
Telephone: +86-551-5161140
Received: September 9, 2003 Revised: October 5, 2003 Accepted: October 12, 2003 Published online: July 1, 2004
Abstract
AIM: To analyze loss of heterozygosity (LOH) and homozygous deletion on p53 gene (exon2-3, 4 and 11), chromosome 10q22-10q23 and 22q11.2-22q12.1 in human hepatocellular carcinoma (HCC).
METHODS: PCR and PCR-based microsatellite polymorphism analysis techniques were used.
RESULTS: LOH was observed at D10S579 (10q22-10q23) in 4 of 20 tumors (20%), at D22S421 (22q11.2-22q12.1) in 3 of 20 (15%), at TP53.A (p53 gene exon 2-3) in 4 of 20 (20%), at TP53.B (p53 gene exon 4) in 6 of 20 (30%), and at TP53.G (p53 gene exon 11) in 0 of 20 (0%). Homozygous deletion was detected at 10q22-10q23 (8/20; 40%), 22q11.2-22q12.1 (8/20; 40%), p53 gene exon 2-3 (0/20;0%), p53 gene exon 4 (6/20; 30%), and p53 gene exon 11 (2/20; 10%).
CONCLUSION: There might be unidentified tumor suppressor genes on chromosome 10q22-10q23 and 22q11.2-22q12.1 that contribute to the pathogenesis and development of HCC.
