Myeloperoxidase, extracellular DNA and neutrophil extracellular trap formation in the animal models of metabolic dysfunction-associated steatotic liver disease

doi:10.3748/wjg.v31.i27.106166

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Basic Study

World J Gastroenterol. Jul 21, 2025; 31(27): 106166
Published online Jul 21, 2025. doi: 10.3748/wjg.v31.i27.106166

Myeloperoxidase, extracellular DNA and neutrophil extracellular trap formation in the animal models of metabolic dysfunction-associated steatotic liver disease

Andrej Feješ, Paulína Belvončíková, Emil Bečka, Tomáš Strečanský, Michal Pastorek, Jakub Janko, Barbora Filová, Pavel Babál, Katarína Šebeková, Veronika Borbélyová, Roman Gardlík

Andrej Feješ, Paulína Belvončíková, Emil Bečka, Tomáš Strečanský, Michal Pastorek, Jakub Janko, Katarína Šebeková, Veronika Borbélyová, Roman Gardlík, Institute of Molecular Biomedicine, Comenius University Faculty of Medicine, Bratislava 81108, Slovakia

Barbora Filová, Institute of Histology and Embryology, Comenius University Faculty of Medicine, Bratislava 81108, Slovakia

Pavel Babál, Institute of Pathological Anatomy, Comenius University Faculty of Medicine, Bratislava 81108, Slovakia

Author contributions: Belvončíková P, Feješ A and Gardlík R conceived and designed research; Belvončíková P, Feješ A, Bečka E, Strečanský T, Janko J, Filová B and Pastorek M performed experiments; Bečka E, Strečanský T and Šebeková K analyzed data; Šebeková K, Babál P, Pastorek M and Gardlík R interpreted results of experiments; Feješ A prepared figures; Feješ A and Belvončíková P drafted manuscript; Babál P, Borbélyová V and Gardlík R edited and revised manuscript; Gardlík R approved final version of manuscript; All authors have read and approved the final manuscript.

Supported by Slovak Research and Development Agency, No. APVV-21-0370; and Ministry of Education, Science, Research and Sport of the Slovak Republic, No. VEGA 1/0706/25 and No. VEGA 1/0341/23.

Institutional review board statement: The study was reviewed and approved by the Institute of Pathophysiology, Faculty of Medicine, Comenius University Institutional Review Board (SK U 06021 Approval No. 07/2023).

Institutional animal care and use committee statement: All procedures involving animals were reviewed and approved by the Institutional Animal Care and Use Committee of the State Veterinary and Food Administration of the Slovak Republic (IACUC protocol number: 4503-3/2024-220).

Conflict-of-interest statement: The authors declare no conflicts of interest.

ARRIVE guidelines statement: The authors have read the ARRIVE Guidelines, and the manuscript was prepared and revised according to the ARRIVE Guidelines.

Data sharing statement: Technical appendix, statistical code, and dataset available from the corresponding author at roman.gardlik@fmed.uniba.sk. No additional data are available.

Open Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/

Corresponding author: Roman Gardlík, MD, PhD, Associate Professor, Head, Institute of Molecular Biomedicine, Comenius University Faculty of Medicine, Sasinkova 4, Bratislava 81108, Slovakia. roman.gardlik@fmed.uniba.sk

Received: February 25, 2025
Revised: June 12, 2025
Accepted: June 16, 2025
Published online: July 21, 2025
Processing time: 146 Days and 21 Hours

Core Tip

Core Tip: Metabolic dysfunction-associated steatotic liver disease (MASLD) is a growing global health concern with limited therapeutic options. This study demonstrated that extracellular DNA (ecDNA) and neutrophil extracellular traps (NETs) serve as potential biomarkers of liver injury severity in MASLD. Using three distinct animal models, we showed that NET markers, including myeloperoxidase and ecDNA, correlated with liver fibrosis and inflammation. These findings highlighted NETs and ecDNA as promising targets for early diagnosis and intervention in MASLD, offering new insights into disease progression and potential therapeutic strategies.