Basic Study
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World J Gastroenterol. Mar 28, 2025; 31(12): 103991
Published online Mar 28, 2025. doi: 10.3748/wjg.v31.i12.103991
Increased keratin 80 expression predicts poor prognosis and promotes oxaliplatin resistance in gastric cancer
Bo Yang, Hong-Xia Cao, Ya-Wei He, Ji-Jie Ouyang, Meng Lv, Yong-Xiang Li, Yi-Da Lu
Bo Yang, Yong-Xiang Li, Department of General Surgery, The First Affiliated Hospital of Anhui Medical University, Hefei 230022, Anhui Province, China
Hong-Xia Cao, Department of Gastroenterology, The Second Affiliated Hospital of Anhui Medical University, Hefei 230601, Anhui Province, China
Ya-Wei He, Ji-Jie Ouyang, Meng Lv, Yi-Da Lu, Department of General Surgery, The Second Affiliated Hospital of Anhui Medical University, Hefei 230601, Anhui Province, China
Co-first authors: Bo Yang and Hong-Xia Cao.
Co-corresponding authors: Yong-Xiang Li and Yi-Da Lu.
Author contributions: Lu YD and Yang B conceptualized and designed the research; Cao HX screened patients and acquired clinical data; He YW, Lv M and Ouyang JJ performed cell function experiments and clinical data compilation; Lu YD and Li YX wrote the paper; All authors have read and approved the manuscript. Yang B proposed, designed and performed data analysis. Cao HX was responsible for patient screening, enrollment, collection of clinical data and tissue specimens. Both authors have made crucial and indispensable contributions towards the completion of the project and thus qualified as the co-first authors of the paper. Lu YD and Li YX have played important and indispensable roles in the experimental design, data interpretation and manuscript preparation as the co-corresponding authors.
Supported by National Natural Science Foundation of China, No. 874063.
Institutional review board statement: The study was carried out in accordance with relevant guidelines and regulations. This present study was approved by the Institute Biomedical Research Ethics Committee of Anhui Medical University (No. YX2024-122) and written informed consent was obtained from all patients involved.
Institutional animal care and use committee statement: All experiments involving animals follow the guidelines of the Animal Center of Anhui Medical University, and all animal experimental protocols were approved by the experimental Animal Ethical Committee of Anhui Medical University, No. LLSC20180345.
Conflict-of-interest statement: All the authors report no relevant conflicts of interest for this article.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Data sharing statement: Data supporting the conclusions of this article are available upon request to the corresponding author at luyida66@163.com, subject to applicable legal and ethical restrictions.
Open Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Yi-Da Lu, Department of General Surgery, The Second Affiliated Hospital of Anhui Medical University, No. 678 Furong Road, Hefei 230601, Anhui Province, China. luyida66@163.com
Received: December 9, 2024
Revised: January 26, 2025
Accepted: February 24, 2025
Published online: March 28, 2025
Processing time: 110 Days and 15.7 Hours
Core Tip

Core Tip: In this paper, keratin 80 (KRT80) was found to be overexpressed in gastric cancer (GC) tissues, functioning as an oncogene and correlating with poor patient prognosis. KRT80 promoted GC proliferation, migration, invasion abilities and oxaliplatin resistance by activating the NF-κB signaling pathway. Immunoprecipitation and mass spectrometry analyses identified elongation factor 1-alpha 1 (EEF1A1) as a binding protein of KRT80, enhancing its protein stability. Notably, knockdown of EEF1A1 partially reversed the effects of KRT80 overexpression in GC cells.