Basic Study
Copyright ©The Author(s) 2025. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Mar 28, 2025; 31(12): 103094
Published online Mar 28, 2025. doi: 10.3748/wjg.v31.i12.103094
scRNA-seq of the intestine reveals the key role of mast cells in early gut dysfunction associated with acute pancreatitis
Zu-Xing Wei, Shi-He Jiang, Xiao-Yan Qi, Yi-Miao Cheng, Qiong Liu, Xu-Yang Hou, Jun He
Zu-Xing Wei, Xiao-Yan Qi, Yi-Miao Cheng, Jun He, Department of General Surgery, The Second Xiangya Hospital, Central South University, Changsha 410011, Hunan Province, China
Shi-He Jiang, Department of Pathology, The Second Xiangya Hospital, Central South University, Changsha 410011, Hunan Province, China
Qiong Liu, Department of Stomatology, The Second Xiangya Hospital, Central South University, Changsha 410011, Hunan Province, China
Xu-Yang Hou, Department of Cardiovascular Surgery, The Second Xiangya Hospital, Central South University, Changsha 410011, Hunan Province, China
Author contributions: He J designed the study; Wei ZX performed most of experiments, analyzed the data and wrote the manuscript; Jiang SH, Qi XY, Cheng YM, Liu Q, and Hou XY assisted with the experiments.
Supported by National Natural Science Foundation of China, No. 82300739; Hunan Provincial Natural Science Foundation, No. 2023JJ40821; and Changsha Natural Science Foundation, No. kq2208308.
Institutional animal care and use committee statement: All animal experiments employed appropriate measures to minimize pain or discomfort, and the experimental protocols were approved by the Animal Ethics Committee of Xiangya Second Hospital, Central South University (Approval No. 20200890).
Conflict-of-interest statement: The authors declare no conflict of interests.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Data sharing statement: The data that support the findings of this study are available from the corresponding author upon reasonable request. Datasets are accessible directly via email communication with the corresponding author.
Open Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Jun He, MD, Department of General Surgery, The Second Xiangya Hospital, Central South University, No. 139 Renmin Road, Changsha 410011, Hunan Province, China. junjunhe@csu.edu.cn
Received: November 10, 2024
Revised: January 9, 2025
Accepted: February 21, 2025
Published online: March 28, 2025
Processing time: 137 Days and 20 Hours
Core Tip

Core Tip: Our study provides a comprehensive picture of the transcriptome of small intestine cells during the early stage of acute pancreatitis, revealing a total of 33232 cells across all samples and 17 main clusters. Through our investigation, we established that mast cells (MCs) were promptly activated in the intestine, and we identified CCL5 derived from MCs as an indispensable factor contributing to the infiltration of inflammatory cells and the progression of gut barrier dysfunction.