Basic Study
Copyright ©The Author(s) 2022. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Aug 7, 2022; 28(29): 3869-3885
Published online Aug 7, 2022. doi: 10.3748/wjg.v28.i29.3869
N-linked glycoproteomic profiling in esophageal squamous cell carcinoma
Qi-Wei Liu, Hao-Jie Ruan, Wei-Xia Chao, Meng-Xiang Li, Ye-Lin Jiao, Douglas G Ward, She-Gan Gao, Yi-Jun Qi
Qi-Wei Liu, Hao-Jie Ruan, Wei-Xia Chao, Meng-Xiang Li, She-Gan Gao, Yi-Jun Qi, Henan Key Laboratory of Microbiome and Esophageal Cancer Prevention and Treatment; Henan Key Laboratory of Cancer Epigenetics; Cancer Hospital, The First Affiliated Hospital, College of Clinical Medicine, Henan University of Science and Technology, Luoyang 471003, Henan Province, China
Ye-Lin Jiao, Department of Pathology, The First People’s Hospital of Luo Yang, Luoyang 471000, Henan Province, China
Douglas G Ward, Institute of Cancer and Genomic Sciences, College of Medical and Dental Sciences, University of Birmingham, Birmingham B15 2TT, United Kingdom
Author contributions: Qi YJ and Gao SG designed and coordinated the study; Liu QW, Ruan HJ, Chao WX, Li MX, Jiao YL, and Ward DG performed the experiments, and acquired and analyzed the data; Qi YJ and Ward DG wrote the manuscript; and all authors approved the final version of the article.
Supported by National Natural Science Foundation of China, No. 81072039 and No. 81872037.
Institutional review board statement: The study was approved by the Ethics Committee of the Medical School, Henan University, China (ethics ref: 108) and conducted in accordance with the ethical guidelines of the 1975 Declaration of Helsinki.
Conflict-of-interest statement: All authors report no relevant conflicts of interest for this article.
Data sharing statement: No additional data are available.
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Yi-Jun Qi, MD, PhD, Professor, Henan Key Laboratory of Microbiome and Esophageal Cancer Prevention and Treatment; Henan Key Laboratory of Cancer Epigenetics; Cancer Hospital, The First Affiliated Hospital, College of Clinical Medicine, Henan University of Science and Technology, No. 29 Jinghua Road, Luoyang 471003, Henan Province, China. qiyijun@haust.edu.cn
Received: January 14, 2022
Peer-review started: January 14, 2022
First decision: April 12, 2022
Revised: April 26, 2022
Accepted: July 6, 2022
Article in press: July 6, 2022
Published online: August 7, 2022
Processing time: 200 Days and 16.2 Hours
Core Tip

Core Tip: The N-linked glycoproteome was comprehensively profiled by two-dimensional gel electrophoresis-based and isobaric tags for relative and absolute quantification (iTRAQ) labeling-based mass spectrometry quantitation in parallel after N-linked glycoprotein enrichment by a tandem of multilectin affinity chromatography. The iTRAQ labeling-based quantitative proteomic profiling outperformed protein spot intensity quantification used by two-dimensional gel electrophoresis. A total of 411 N-linked glycoproteins were identified, including 128 up-regulated and 283 down-regulated glycoproteins with differential expression, which provide the scientific community with a dataset of glycoproteins associated with esophageal squamous cell carcinoma for in-depth investigation.