Basic Study
Copyright ©The Author(s) 2021. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Sep 7, 2021; 27(33): 5575-5594
Published online Sep 7, 2021. doi: 10.3748/wjg.v27.i33.5575
Characterization of gut microbiome and metabolome in Helicobacter pylori patients in an underprivileged community in the United States
Brian White, John D Sterrett, Zoya Grigoryan, Lauren Lally, Jared D Heinze, Hyder Alikhan, Christopher A Lowry, Lark J Perez, Joshua DeSipio, Sangita Phadtare
Brian White, Sangita Phadtare, Department of Biomedical Sciences, Cooper Medical School of Rowan University, Camden, NJ 08103, United States
John D Sterrett, Jared D Heinze, Christopher A Lowry, Department of Integrative Physiology, University of Colorado Boulder, Boulder, CO 80309, United States
Zoya Grigoryan, Department of Internal Medicine, Lenox Hill Hospital, NYC, NY 10075, United States
Lauren Lally, Department of Internal Medicine, Thomas Jefferson University Hospital, Philadelphia, PA 19107, United States
Hyder Alikhan, Department of Biological Sciences, Rowan University, Glassboro, NJ 08028, United States
Lark J Perez, Department of Chemistry and Biochemistry, Rowan University, Glassboro, NJ 08028, United States
Joshua DeSipio, Department of Gastroenterology, Cooper University Hospital, Camden, NJ 08103, United States
Author contributions: White B, Sterrett JD and Grigoryan Z contributed equally to the work; DeSipio J, and Phadtare S designed and coordinated the study; Grigoryan Z and Lally L collected patient samples and data; Phadtare S performed the sample processing for 16S rRNA gene sequencing; Heinze JD provided guidance for sample processing methodology; Grigoryan Z and Phadtare S performed the 16S rRNA sequencing data analysis; Sterrett JD and Lowry CA created the analysis codes essential for analysis and provided guidance for data analysis; Alikhan H and Perez LJ performed the fatty acid analysis; Lally L and White B carried out literature search; White B, Sterrett JD, Lowry CA, Perez LJ, DeSipio J and Phadtare S interpreted the analyzed data and wrote the final draft of the manuscript; all authors approved the final version of the article.
Supported by The Camden Health Research Initiative grant to Sangita Phadtare.
Institutional review board statement: This study received approval by the Cooper Health System Institutional Review Board (IRB) (17-077EX) and all the steps were carried out as per the standards set by the IRB.
Institutional animal care and use committee statement: There are no animals used in the study.
Conflict-of-interest statement: The authors report no conflicts of interest.
Data sharing statement: The code is available at https://github.com/sterrettJD/H-pylori-microbiome-analysis. Volcano plot data are included as Supplementary Tables 1-3(Supplementary Table 1: Volcano plot data with all Helicobacter data set; Supplementary Table 2: Volcano plot data with High Bacteroidetes; Supplementary Table 3: Volcano plot data with High Firmicutes).
Open-Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Sangita Phadtare, BSc, MSc, PhD, Professor, Department of Biomedical Sciences, Cooper Medical School of Rowan University, 401 S Broadway, Camden, NJ 08103, United States. phadtare@rowan.edu
Received: March 31, 2021
Peer-review started: March 31, 2021
First decision: June 23, 2021
Revised: July 2, 2021
Accepted: August 13, 2021
Article in press: August 13, 2021
Published online: September 7, 2021
Processing time: 155 Days and 15.9 Hours
Core Tip

Core Tip: Helicobacter pylori (H. pylori) infects half of the world’s population and is associated with various diseases, including malignancy. Research of microbiome and metabolomic changes associated with H. pylori may hold therapeutic potential. We sought to characterize the fecal microbiome and fatty acid metabolism among H. pylori patients in our community. We observed differences in alpha and beta diversity among H. pylori patients compared to controls, particularly for those over 40 years old. Changes in several fecal fatty acids, including those associated with anti-inflammatory activity, were observed. Our findings may have implications for improving H. pylori eradication and minimizing associated negative distal outcomes.