Alharbi SA, Ovchinnikov DA, Wolvetang E. Leucine-rich repeat-containing G protein-coupled receptor 5 marks different cancer stem cell compartments in human Caco-2 and LoVo colon cancer lines. World J Gastroenterol 2021; 27(15): 1578-1594 [PMID: 33958845 DOI: 10.3748/wjg.v27.i15.1578]
Corresponding Author of This Article
Samah Abdulaali Alharbi, PhD, Assistant Professor, Senior Scientist, Physiology Department, College of Medicine, Umm Al-Qura University, Prince Sultan bin AbdulAziz road, Makkah 24231, Saudi Arabia. saaharbi@uqu.edu.sa
Research Domain of This Article
Gastroenterology & Hepatology
Article-Type of This Article
Basic Study
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Alharbi SA, Ovchinnikov DA, Wolvetang E. Leucine-rich repeat-containing G protein-coupled receptor 5 marks different cancer stem cell compartments in human Caco-2 and LoVo colon cancer lines. World J Gastroenterol 2021; 27(15): 1578-1594 [PMID: 33958845 DOI: 10.3748/wjg.v27.i15.1578]
World J Gastroenterol. Apr 21, 2021; 27(15): 1578-1594 Published online Apr 21, 2021. doi: 10.3748/wjg.v27.i15.1578
Leucine-rich repeat-containing G protein-coupled receptor 5 marks different cancer stem cell compartments in human Caco-2 and LoVo colon cancer lines
Samah Abdulaali Alharbi, Dmitry A Ovchinnikov, Ernst Wolvetang
Samah Abdulaali Alharbi, Physiology Department, College of Medicine, Umm Al-Qura University, Makkah 24231, Saudi Arabia
Samah Abdulaali Alharbi, Ernst Wolvetang, Department of Stem Cell Engineering Group, Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane 4072, QLD, Australia
Dmitry A Ovchinnikov, Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Brisbane 4072, QLD, Australia
Author contributions: Alharbi SA contributed investigation, methodology, formal analysis, visualization, preparation and original draft preparation; Ovchinnikov DA contributed conceptualization, methodology and supervision; Wolvetang E contributed conceptualization, supervision, funding, acquisition, project administration and resources and reviewed and edited the manuscript.
Institutional review board statement: The study was reviewed and approved by the Institutional Review Board at The University of Queensland (approval No. 2019000159).
Institutional animal care and use committee statement: All animal experiments conformed to the internationally accepted principles for the care and use of laboratory animals, The Australian Institute of Bioengineering and Nanotechnology, Brisbane; Australia (approval No. AIBN/065/12/SCA/LEJEUNE/KACST).
Conflict-of-interest statement: All authors have nothing to disclose.
Data sharing statement: No additional data are available.
ARRIVE guidelines statement: The authors have read the ARRIVE Guidelines, and the manuscript was prepared and revised according to the ARRIVE Guidelines.
Corresponding author: Samah Abdulaali Alharbi, PhD, Assistant Professor, Senior Scientist, Physiology Department, College of Medicine, Umm Al-Qura University, Prince Sultan bin AbdulAziz road, Makkah 24231, Saudi Arabia. saaharbi@uqu.edu.sa
Received: December 1, 2020 Peer-review started: December 1, 2020 First decision: December 21, 2020 Revised: January 22, 2021 Accepted: February 24, 2021 Article in press: February 24, 2021 Published online: April 21, 2021 Processing time: 133 Days and 18.6 Hours
Core Tip
Core Tip: The intestinal epithelium harbors two distinct pools of putative stem cells, the leucine-rich repeat-containing G protein-coupled receptor 5+ (LGR5) stem cell population and the B lymphoma Moloney murine leukemia virus insertion region 1 homolog+ stem cell population. Colon cancer cell lines such as Caco-2 and LoVo are extensively used in colon cancer research, and express high levels of LGR5. Here, we aimed to investigate whether colon cancer cell lines contain cancer stem cells and characterized these cells. Using an LGR5 reporter, we characterized LGR5+ cells and revealed that Caco-2 cell line contains a classical intestinal stem cell-like population (LGR5+). However, in LoVo cell lines, stem cell-like population is more akin to the B lymphoma Moloney murine leukemia virus insertion region 1 homolog+ stem cells.
