©The Author(s) 2019. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Feb 7, 2019; 25(5): 584-599
Published online Feb 7, 2019. doi: 10.3748/wjg.v25.i5.584
Published online Feb 7, 2019. doi: 10.3748/wjg.v25.i5.584
Claudin-7 gene knockout causes destruction of intestinal structure and animal death in mice
Chang Xu, Kun Wang, Yu-Han Ding, Wen-Jing Li, Lei Ding, Department of Oncology, Beijing Shijitan Hospital, Capital Medical University, Beijing 100038, China
Author contributions: Ding L designed the study; Xu C performed the research and wrote the paper; Wang K and Ding YH fed the mice; Li WJ analysed the data.
Supported by the National Natural Science Foundation of China , No. 81372585 and No. 81772557 ; and Beijing Health System High Level Training Plan of Health Technical Personnel , No. 2014-3-048 .
Institutional review board statement: The study was reviewed and approved by the Medical Ethics Committee of the Capital Medical University Affiliated Beijing Shijitan Hospital Institutional Review Board.
Institutional animal care and use committee statement: All protocols were carried out in accordance with relevant guidelines and regulations.
Conflict-of-interest statement: The authors declare no conflict of interest.
Data sharing statement: No additional data are available.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Corresponding author: Lei Ding, MD, PhD, Associate Professor, Chief Doctor, Surgeon, Surgical Oncologist, Department of Oncology, Beijing Shijitan Hospital, Capital Medical University, No. 10, Tieyi Road, Haidian District, Beijing 100038, China. dinglei1005@126.com
Telephone: +86-10-63926296 Fax: +86-10-63926296
Received: October 24, 2018
Peer-review started: October 25, 2018
First decision: November 29, 2018
Revised: January 10, 2019
Accepted: January 18, 2019
Article in press: January 18, 2019
Published online: February 7, 2019
Processing time: 99 Days and 1.3 Hours
Peer-review started: October 25, 2018
First decision: November 29, 2018
Revised: January 10, 2019
Accepted: January 18, 2019
Article in press: January 18, 2019
Published online: February 7, 2019
Processing time: 99 Days and 1.3 Hours
Core Tip
Core tip: The intestinal tract of conventional and intestinal specific Claudin-7 knockout mice was characterized by extensive and severe inflammation. The development of inducible conditional knockout mice can control the knockout of Claudin-7 in a temporal and compartment specific manner and prolong the survival time of mice, which exhibited atypical hyperplasia and adenoma in the intestine. This study revealed the inhibitory role that Claudin-7 plays in colorectal inflammation and colorectal cancer.