Basic Study
Copyright ©The Author(s) 2018. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Nov 21, 2018; 24(43): 4880-4892
Published online Nov 21, 2018. doi: 10.3748/wjg.v24.i43.4880
Establishment, functional and genetic characterization of three novel patient-derived rectal cancer cell lines
Michael Gock, Christina S Mullins, Carina Bergner, Friedrich Prall, Robert Ramer, Anja Göder, Oliver H Krämer, Falko Lange, Bernd J Krause, Ernst Klar, Michael Linnebacher
Michael Gock, Ernst Klar, Department of General Surgery, University Medical Center, Rostock 18055, Germany
Christina S Mullins, Michael Linnebacher, Section of Molecular Oncology and Immunotherapy, University Medical Center, Rostock 18055, Germany
Carina Bergner, Bernd J Krause, Department of Nuclear Medicine, University Medical Center, Rostock 18055, Germany
Friedrich Prall, Institute of Pathology, University Medical Center, Rostock 18055, Germany
Robert Ramer, Institute of Pharmacology, University Medical Center, Rostock 18055, Germany
Anja Göder, Oliver H Krämer, Institute of Toxicology, University Medical Center Mainz, Mainz 55131, Germany
Falko Lange, Oscar-Langendorff-Institute of Physiology, University Medical Center, Rostock 18055, Germany
Author contributions: Gock M, Lange F, Krause BJ and Linnebacher M designed research; Mullins CS, Prall F, Ramer R, Göder A and Lange F performed research; Gock M, Prall F, Krämer OH, Lange F, Krause BJ and Linnebacher M analyzed and reviewed data; Gock M, Bergner C and Klar E provided materials necessary to perform the experiments; Gock M and Linnebacher M wrote the manuscript; Mullins CS, Krämer OH, Lange F and Krause BJ contributed parts of the manuscript and critically reviewed the final version of the manuscript.
Supported by the German Research Foundation to Oliver H Krämer, No. KR 2291/7-1.
Institutional animal care and use committee statement: All procedures were approved by Ethikkommission an der Medizinischen Fakultät der Universität Rostock (reference number II HV 43/2004) in accordance with generally accepted guidelines for the use of human material.
Conflict-of-interest statement: To the best of our knowledge, no conflict of interest exists.
Data sharing statement: No additional data to share.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Institutional review board statement: This study was reviewed and approved by Ethikkommission an der Medizinischen Fakultät der Universität Rostock, Germany.
Correspondence to: Michael Linnebacher, PhD, Postdoc, Research Scientist, Department of General Surgery, Section of Molecular Oncology and Immunotherapy, University Medical Center, Schillingallee 35, Rostock 18055, Germany. michael.linnebacher@med.uni-rostock.de
Telephone: +49-381-4946043 Fax: +49-381-4946002
Received: August 3, 2018
Peer-review started: August 3, 2018
First decision: October 8, 2018
Revised: October 22, 2018
Accepted: November 2, 2018
Article in press: November 2, 2018
Published online: November 21, 2018
Processing time: 110 Days and 14.1 Hours
Core Tip

Core tip: Ultra-low passage and in-depth characterized tumor models are highly desirable for basic research and assessment of individual response prediction to current or novel therapy regimens. Here, for the first time, we describe three patient-derived rectal cancer cell lines established either directly from patient’s tumor samples or after xenografting. These tumor models were characterized according to phenotype, molecular-, as well as growth and morphological features and sensitivity to chemotherapeutic drugs and radiation, including radiochemotherapy. In addition, glucose metabolism was assessed with 18F-fluorodeoxyglucose and proliferation with 18F-fluorothymidine. These cell lines provide excellent tools for basic and translational research of rectal cancers’ biological characteristics.