Xie SR, An JY, Zheng LB, Huo XX, Guo J, Shih D, Zhang XL. Effects and mechanism of adenovirus-mediated phosphatase and tension homologue deleted on chromosome ten gene on collagen deposition in rat liver fibrosis. World J Gastroenterol 2017; 23(32): 5904-5912 [PMID: 28932082 DOI: 10.3748/wjg.v23.i32.5904]
Corresponding Author of This Article
Xiao-Lan Zhang, MD, PhD, Professor, Department of Gastroenterology, The Second Hospital of Hebei Medical University, Hebei Key Laboratory of Gastroenterology, Hebei Institute of Gastroenterology, 215 West Heping Road, Shijiazhuang 050000, Hebei Province, China. xiaolanzh@hb2h.com
Research Domain of This Article
Gastroenterology & Hepatology
Article-Type of This Article
Basic Study
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Xie SR, An JY, Zheng LB, Huo XX, Guo J, Shih D, Zhang XL. Effects and mechanism of adenovirus-mediated phosphatase and tension homologue deleted on chromosome ten gene on collagen deposition in rat liver fibrosis. World J Gastroenterol 2017; 23(32): 5904-5912 [PMID: 28932082 DOI: 10.3748/wjg.v23.i32.5904]
World J Gastroenterol. Aug 28, 2017; 23(32): 5904-5912 Published online Aug 28, 2017. doi: 10.3748/wjg.v23.i32.5904
Effects and mechanism of adenovirus-mediated phosphatase and tension homologue deleted on chromosome ten gene on collagen deposition in rat liver fibrosis
Xiao-Lan Zhang, David Shih, Jian Guo, Xiao-Xia Huo, Li-Bo Zheng, Jun-Yan An, Shu-Rui Xie
Shu-Rui Xie, Jun-Yan An, Li-Bo Zheng, Xiao-Xia Huo, Jian Guo, Xiao-Lan Zhang, Department of Gastroenterology, The Second Hospital of Hebei Medical University, Hebei Key Laboratory of Gastroenterology, Hebei Institute of Gastroenterology, Shijiazhuang 050000, Hebei Province, China
Shu-Rui Xie, Department of Gastroenterology, Xingtai People's Hospital, Xingtai 054031, Hebei Province, China
David Shih, Inflammatory Bowel and Immunobiology Research Institute, F. Widjaja Foundation, Cedars-Sinai Medical Center, Los Angeles, CA 90048, United States
Author contributions: Xie SR and An JY contributed equally to this work; Xie SR and An JY performed the majority of the experiments; Zheng LB and Guo J assisted with various experiments and helped to analyze the data; Huo XX and Zhang XL drafted and edited the manuscript; Shih D performed critical revision of the manuscript.
Supported bythe National Natural Science Foundation of China, No. 30872513.
Institutional review board statement: This study was reviewed and approved by the Second Hospital of Hebei Medical University Institutional Review Board.
Institutional animal care and use committee statement: All procedures involving animals were reviewed and approved by the Institutional Animal Care and Use Committee of the Experimental Animal Center of Hebei Medical University (No. 911102).
Conflict-of-interest statement: We declare that there are no conflicts of interest to disclose.
Data sharing statement: Technical appendix, statistical code, and dataset available from the corresponding author at xiaolanzh@126.com. Participants gave informed consent for data sharing.
Correspondence to: Xiao-Lan Zhang, MD, PhD, Professor, Department of Gastroenterology, The Second Hospital of Hebei Medical University, Hebei Key Laboratory of Gastroenterology, Hebei Institute of Gastroenterology, 215 West Heping Road, Shijiazhuang 050000, Hebei Province, China. xiaolanzh@hb2h.com
Telephone: +86-311-66007370 Fax: +86-311-66007370
Received: January 26, 2017 Peer-review started: February 4, 2017 First decision: April 21, 2017 Revised: June 19, 2017 Accepted: July 22, 2017 Article in press: July 24, 2017 Published online: August 28, 2017 Processing time: 213 Days and 8.5 Hours
Core Tip
Core tip: Phosphatase and tension homologue deleted on chromosome ten (PTEN) has a negative relation with the activation and proliferation of hepatic stellate cells (HSCs), which is the central event in liver fibrogenesis as HSCs are the major source of collagens and matrix metalloproteinases in fibrotic liver. In this study, adenoviruses containing cDNA constructs encoding wild-type PTEN (Ad-PTEN) and PTEN mutant G129E gene (Ad-G129E) were constructed to over-express the PTEN gene in both rat primary HSCs and human LX-2 cells as well as in the CCl4-induced rat liver fibrosis model. The adenovirus-mediated over-expression of the PTEN gene attenuated extracellular matrix (ECM) synthesis (collagens I and III) and promoted ECM degradation, representing a possible novel anti-fibrosis therapy.
