Zheng XY, Lv YF, Li S, Li Q, Zhang QN, Zhang XT, Hao ZM. Recombinant adeno-associated virus carrying thymosin β4 suppresses experimental colitis in mice. World J Gastroenterol 2017; 23(2): 242-255 [PMID: 28127198 DOI: 10.3748/wjg.v23.i2.242]
Corresponding Author of This Article
Zhi-Ming Hao, MD, PhD, Professor, Department of Rheumatology, The First Affiliated Hospital of Xi’an Jiaotong University, 277 Yantaxilu, Xi’an 710061, Shaanxi Province, China. haozhm66@126.com
Research Domain of This Article
Gastroenterology & Hepatology
Article-Type of This Article
Basic Study
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Xiao-Yan Zheng, Qian Li, Zhi-Ming Hao, Department of Rheumatology, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710061, Shaanxi Province, China
Yi-Fei Lv, Department of Gastroenterology, Shaanxi Provincial People’s Hospital, Xi’an 710061, Shaanxi Province, China
Shuang Li, Qian-Nan Zhang, Xue-Ting Zhang, Department of Gastroenterology, The First Affiliated Hospital of Xi’an Jiaotong University, Xi’an 710061, Shaanxi Province, China
Author contributions: Zheng XY and Hao ZM designed and coordinated the research; Li S and Zhang XT prepared the recombinant AAVs; Zheng XY, Lv YF and Zhang QN performed the animal experiments; Zheng XY, Lv YF, Li Q and Zhang QN performed the immunohistochemistry, TUNEL, ELISA and Western blot; Zheng XY and Li Q analyzed the data; Zheng XY and Hao ZM wrote the manuscript.
Supported byNational Foundation of Natural Sciences, China, No. 81300293.
Institutional review board statement: The study was reviewed and approved by the Institutional Animal Ethics Committee of Xi’an Jiaotong University, Xi’an, Shaanxi Province, China (Permit No. XJTULAC2016-404).
Institutional animal care and use committee statement: All procedures involving animals were reviewed and approved by the Institutional Animal Care and Use Committee of Xi’an Jiaotong University, Xi’an, Shaanxi Province, China.
Conflict-of-interest statement: The authors declare that they have no conflict of interest to disclose concerning this manuscript.
Data sharing statement: No additional data are available.
Correspondence to: Zhi-Ming Hao, MD, PhD, Professor, Department of Rheumatology, The First Affiliated Hospital of Xi’an Jiaotong University, 277 Yantaxilu, Xi’an 710061, Shaanxi Province, China. haozhm66@126.com
Telephone: +86-18991232223
Received: June 25, 2016 Peer-review started: June 28, 2016 First decision: September 6, 2016 Revised: October 4, 2016 Accepted: November 13, 2016 Article in press: November 13, 2016 Published online: January 14, 2017 Processing time: 201 Days and 3.1 Hours
Core Tip
Core tip: We confirmed that intracolonically administered recombinant adeno-associated virus (AAVs) efficiently mediated the ectopic expression of LacZ and thymosin β4 (Tβ4) in mouse colonic mucosa. The current study first indicated that AAV-Tβ4 could improve murine colitis induced either by dextran sulfate sodium or 2,4,6-trinitrobenzene sulfonic acid by suppressing inflammatory cell infiltration, alleviating oxidative stress and epithelial apoptosis, and modulating the production of inflammatory mediators in the inflamed colon. Furthermore, locally overexpressed Tβ4 could attenuate the proliferation of colonic mucosal epithelia. In summary, these results suggest a protective role of Tβ4 in inflammatory bowel diseases (IBD) and indicate that AAV-Tβ4 has therapeutic potential for IBD patients.
