©The Author(s) 2017. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. May 14, 2017; 23(18): 3338-3348
Published online May 14, 2017. doi: 10.3748/wjg.v23.i18.3338
Published online May 14, 2017. doi: 10.3748/wjg.v23.i18.3338
In vivo and ex vivo confocal endomicroscopy of pancreatic cystic lesions: A prospective study
Somashekar G Krishna, Phil A Hart, Darwin L Conwell, Division of Gastroenterology, Hepatology and Nutrition, The Ohio State University Wexner Medical Center, Columbus, OH 43210, United States
Rohan M Modi, Department of Internal Medicine, The Ohio State University Wexner Medical Center, Columbus, OH 43210, United States
Amrit K Kamboj, College of Medicine, The Ohio State University Wexner Medical Center, Columbus, OH 43210, United States
Benjamin J Swanson, Department of Pathology, The Ohio State University Wexner Medical Center, Columbus, OH 43210, United States
Mary E Dillhoff, Carl R Schmidt, Division of Surgical Oncology, The Ohio State University Wexner Medical Center, Columbus, OH 43210, United States
Andrei Manilchuk, Deparment of General Surgery, The Ohio State University Wexner Medical Center, Columbus, OH 43210, United States
Author contributions: Krishna SG designed the protocol, performed all EUS-nCLE and ex vivo CLE procedures, in vivo and ex vivo CLE interpretation, wrote the manuscript, and edited images; Modi RM assisted with background literature, wrote the manuscript, in vivo and ex vivo CLE interpretation, and edited images; Kamboj AK assisted with background literature search, and edited images; Swanson BJ assisted with ex vivo CLE image acquisition, provided pathological interpretation, and edited images; Hart PA performed critical revision of manuscript; Dillhoff ME, Manilchuk A and Schmidt CR performed pancreatic surgeries and provided resected specimens for ex vivo CLE; Conwell DL performed critical revision of manuscript.
Supported by American College of Gastroenterology Pilot Research Grant. The Gastroflex UHD probe for the ex vivo evaluation was provided by Cellvizio, Mauna Kea Technologies, Paris, France .
Institutional review board statement: The study was reviewed and approved by the institutional review boards of The Ohio State University, Columbus, United States.
Clinical trial registration statement: The clinical trial is registered with ClinicalTrials.gov, using identifier NCT02516488. Details can be found at https://clinicaltrials.gov/ct2/show/NCT02516488?term=somashekar&rank=1
Informed consent statement: All study participants, or their legal guardian, provided written consent prior to study enrollment.
Conflict-of-interest statement: None of the authors have any conflicts of interest or financial ties to disclose.
Data sharing statement: There is no additional data available.
Correspondence to: Somashekar G Krishna, MD, MPH, Assistant Professor, Division of Gastroenterology, Hepatology and Nutrition, The Ohio State University Wexner Medical Center, 395 W. 12th Avenue, Suite 262, Columbus, OH 43210, United States. somashekar.krishna@osumc.edu
Telephone: +1-614-2936255 Fax: +1-614-2938518
Received: February 6, 2017
Peer-review started: February 8, 2017
First decision: March 3, 2017
Revised: March 21, 2017
Accepted: April 12, 2017
Article in press: April 12, 2017
Published online: May 14, 2017
Processing time: 98 Days and 5 Hours
Peer-review started: February 8, 2017
First decision: March 3, 2017
Revised: March 21, 2017
Accepted: April 12, 2017
Article in press: April 12, 2017
Published online: May 14, 2017
Processing time: 98 Days and 5 Hours
Core Tip
Core tip: We performed a prospective study to investigate the reproducibility of in vivo endoscopic ultrasound (EUS) - guided needle based confocal endomicroscopy (nCLE) image patterns in an ex vivo setting and compare these to surgical histopathology for pancreatic cystic lesions (PCLs). A total of 10 subjects underwent in vivo EUS-nCLE and subsequently ex vivo CLE of the PCL following surgical resection. Biopsies were obtained from ex vivo CLE-imaged areas for comparative histopathology. We found that characteristic in vivo nCLE patterns were observed during ex vivo pCLE of resected PCLs. Both in vivo and ex vivo CLE imaging findings correlated with surgical histopathology. These findings support the application of EUS-nCLE in the diagnosis of PCLs.