Han J, Li JH, Bai G, Shen GS, Chen J, Liu JN, Wang S, Liu XJ. Acanthopanax senticosus polysaccharides-induced intestinal tight junction injury alleviation via inhibition of NF-κB/MLCK pathway in a mouse endotoxemia model. World J Gastroenterol 2017; 23(12): 2175-2184 [PMID: 28405145 DOI: 10.3748/wjg.v23.i12.2175]
Corresponding Author of This Article
Xian-Jun Liu, PhD, Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, 120 Dongling Road, Shenhe District, Shenyang 110866, Liaoning Province, China. synydoctor@163.com
Research Domain of This Article
Biology
Article-Type of This Article
Basic Study
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Han J, Li JH, Bai G, Shen GS, Chen J, Liu JN, Wang S, Liu XJ. Acanthopanax senticosus polysaccharides-induced intestinal tight junction injury alleviation via inhibition of NF-κB/MLCK pathway in a mouse endotoxemia model. World J Gastroenterol 2017; 23(12): 2175-2184 [PMID: 28405145 DOI: 10.3748/wjg.v23.i12.2175]
World J Gastroenterol. Mar 28, 2017; 23(12): 2175-2184 Published online Mar 28, 2017. doi: 10.3748/wjg.v23.i12.2175
Acanthopanax senticosus polysaccharides-induced intestinal tight junction injury alleviation via inhibition of NF-κB/MLCK pathway in a mouse endotoxemia model
Jie Han, Ji-Hong Li, Guang Bai, Guo-Shun Shen, Jing Chen, Jia-Nan Liu, Shuo Wang, Xian-Jun Liu
Jie Han, Guo-Shun Shen, Jing Chen, Jia-Nan Liu, Xian-Jun Liu, Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, Liaoning Province, China
Ji-Hong Li, Department of Pharmacy, Affiliated Hospital, Liaoning University of Traditional Chinese Medicine, Shenyang 110032, Liaoning Province, China
Guang Bai, Department of Gastroenterology, Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Shenyang 110032, Liaoning Province, China
Shuo Wang, Testing and Analysis Center, Shenyang Agricultural University, Shenyang 110866, Liaoning Province, China
Author contributions: Han J and Liu XJ conceived and designed the experiments; Han J, Li JH, Liu JN and Wang S performed the experiments; Bai G provided proposal of intestinal damage; Shen GS and Chen J statistically analyzed the data; Han J wrote the manuscript; Liu XJ reviewed the manuscript; all authors have read and approved the final manuscript.
Institutional animal care and use committee statement: All procedures involving animals were reviewed and approved by the Institutional Animal Care and Use Committee of College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, China, Protocol No. SYXK (Liao) 2011-0001.
Conflict-of-interest statement: The authors declare that there are no conflicts of interest related to this study.
Data sharing statement: The data referred to in this manuscript have been generated solely by the authors. No other party has been involved. Therefore, no additional unpublished data are available.
Correspondence to: Xian-Jun Liu, PhD, Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, 120 Dongling Road, Shenhe District, Shenyang 110866, Liaoning Province, China. synydoctor@163.com
Telephone: +86-24-88487156 Fax: +86-24-88487156
Received: December 9, 2016 Peer-review started: December 11, 2016 First decision: January 10, 2017 Revised: January 18, 2017 Accepted: February 16, 2017 Article in press: February 17, 2017 Published online: March 28, 2017 Processing time: 108 Days and 23.1 Hours
Core Tip
Core tip:Acanthopanax senticosus polysaccharides (ASPS) effectively protect against gastric tight junction (TJ) injury in sepsis. ASPS pretreatment significantly improved intestinal histological appearance and gut permeability, increased electron dense between adjoining cells, sustained the expression and redistribution of occludin and zonula occludens-1, suppressed the expression of nuclear factor-kappa B p65 (NF-κBp65) and phospho-inhibitor kappa B-alpha and myosin light chain kinase (MLCK), as well as phospho-myosin light-chain-2 in endotoxemia. These findings suggest that ASPS pretreatment may be associated with inhibition of the NF-κB/MLCK pathway and concomitant amelioration of gastric TJ dysfunction in the mouse model of endotoxemia.
