©The Author(s) 2016. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Dec 21, 2016; 22(47): 10341-10352
Published online Dec 21, 2016. doi: 10.3748/wjg.v22.i47.10341
Published online Dec 21, 2016. doi: 10.3748/wjg.v22.i47.10341
Anti-viral role of toll like receptor 4 in hepatitis B virus infection: An in vitro study
Dipanwita Das, Neelakshi Sarkar, Ananya Pal, Debraj Saha, Manikankana Bandopadhyay, Runu Chakravarty, ICMR Virus Unit, Kolkata, ID and BG Hospital Campus, Kolkata 700010, India
Isha Sengupta, Chandrima Das, Biophysics and Structural Genomics Division, Saha Institute of Nuclear Physics, Kolkata 700064, India
Jimmy Narayan, Shivram Prasad Singh, Department of Gastroenterology, SCB Medical College, Cuttack 753007, India
Shivram Prasad Singh, Kalinga Gastroenterology Foundation, Beam Diagnostics Premises, Cuttack 753001, India
Author contributions: Das D, Sarkar N, Das C and Chakravarty R conceived and designed the experiments; Das D, Sarkar N, Sengupta I and Pal A performed the experiments; Das D, Sarkar N, Sengupta I, Das C, Narayan J, Singh SP and Chakravarty R analyzed the data; Das D, Sarkar N, Pal A, Saha D, Bandopadhyay M, Das C and Chakravarty R contributed reagents/materials/analysis tools; Das D, Sarkar N, Sengupta I, Pal A, Saha D, Bandopadhyay M, Das C and Chakravarty R wrote the paper.
Supported by the Indian Council of Medical Research (ICMR) intramural project; Das C acknowledges the financial support from Biomolecular Assembly, Recognition and Dynamics (BARD) project (Grant 12-R &D- SIN-5.04-0103) from Department of Atomic Energy (DAE), Government of India and Ramalingaswami Fellowship, Department of Biotechnology, Government of India .
Institutional review board statement: Signed informed consent was obtained from patients and the study was approved by the institutional ethical committee of Kalinga Gastroenterology Foundation.
Conflict-of-interest statement: The authors declare no conflict of interest.
Correspondence to: Dr. Runu Chakravarty, ICMR Virus Unit, Kolkata, ID and BG Hospital Campus, GB 4, Kolkata 700010, India. runugc@gmail.com
Telephone: +91-33-23537425 Fax: +91-33-23537424
Received: June 26, 2016
Peer-review started: June 27, 2016
First decision: August 29, 2016
Revised: October 1, 2016
Accepted: November 16, 2016
Article in press: November 16, 2016
Published online: December 21, 2016
Processing time: 176 Days and 19.8 Hours
Peer-review started: June 27, 2016
First decision: August 29, 2016
Revised: October 1, 2016
Accepted: November 16, 2016
Article in press: November 16, 2016
Published online: December 21, 2016
Processing time: 176 Days and 19.8 Hours
Core Tip
Core tip: The study delves into the role of toll like receptor 4 (TLR4) in hepatitis B virus (HBV) infection. Inciting TLR4 brings about drastic changes in viral and host responses in hepatocyte niche. Stimulating TLR4 represses viral replication and alters important host gene expression. Virus induced G1/S cell cycle arrest is partially released on viral elimination which is corroborated with the expression of chief cell cycle regulators. Further, we have observed activation of epigenetic signatures H3K9Ac/H3K18Ac, on viral suppression post TLR4 activation. Thus, this work is important in perspective of the role of TLR4 during HBV infection in hepatocyte microenvironment.