Basic Study
Copyright ©The Author(s) 2016. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Apr 21, 2016; 22(15): 3952-3961
Published online Apr 21, 2016. doi: 10.3748/wjg.v22.i15.3952
Gallic acid induced apoptotic events in HCT-15 colon cancer cells
Aruna Priyadharshni Subramanian, Saravana Kumar Jaganathan, Mahitosh Mandal, Eko Supriyanto, Ida Idayu Muhamad
Aruna Priyadharshni Subramanian, Saravana Kumar Jaganathan, Eko Supriyanto, Ida Idayu Muhamad, IJN-UTM Cardiovascular Engineering Centre, Faculty of Biosciences and Medical Engineering, Universiti Teknologi Malaysia, Johor Bahru 81310, Malaysia
Mahitosh Mandal, School of Medical Science and Technology, Indian Institute of Technology, Kharagpur 721302, West Bengal, India
Author contributions: Subramanian AP and Jaganathan SK contributed equally to this work; Jaganathan SK and Mandal M designed the research; Subramanian AP and Jaganathan SK performed the research; Mandal M, Supriyanto E and Muhamad II contributed new reagents/analytic tools; Subramanian AP, Jaganathan SK and Mandal M analyzed the data; and Subramanian AP and Jaganathan SK wrote the paper.
Supported by the Research University Grant scheme with the Grant Vot No. Q.J130000.2509.10H13 (partly).
Conflict-of-interest statement: The authors of the manuscript do not have any conflicts of interest.
Data sharing statement: No additional data are available.
Open-Access: This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Correspondence to: Dr. Saravana Kumar Jaganathan, IJN-UTM Cardiovascular Engineering Centre, Faculty of Biosciences and Medical Engineering, Universiti Teknologi Malaysia, Johor Bahru 81310, Malaysia. jaganathaniitkgp@gmail.com
Telephone: +60-7-5558563 Fax: +60-7-5558553
Received: September 3, 2015
Peer-review started: September 10, 2015
First decision: September 29, 2015
Revised: October 26, 2015
Accepted: December 8, 2015
Article in press: December 8, 2015
Published online: April 21, 2016
Processing time: 208 Days and 5 Hours
Core Tip

Core tip: This article describes the inhibitory effect of gallic acid (GA), against colon cancer cells. GA treatment suppressed the proliferation and colony formation of HCT-15 cells and the anti-cancerous effect of GA was found to follow reactive oxygen species dependent apoptosis. Early events associated with apoptosis like lipid layer breakage and fall in mitochondrial membrane potential were induced by GA treatment in HCT-15 cells along with cell cycle arrest. Further, morphological changes like membrane blebbing and cell shrinkage were seen in the colon cancer cells after GA. Therefore, our results propel the role of GA as a possible anticancer agent.