Proença MA, de Oliveira JG, Cadamuro ACT, Succi M, Netinho JG, Goloni-Bertolo EM, Pavarino &C, Silva AE. TLR2 and TLR4 polymorphisms influence mRNA and protein expression in colorectal cancer. World J Gastroenterol 2015; 21(25): 7730-7741 [PMID: 26167073 DOI: 10.3748/wjg.v21.i25.7730]
Corresponding Author of This Article
Ana Elizabete Silva, PhD, Department of Biology, UNESP, São Paulo State University, Rua Cristóvão Colombo, 2265, São José do Rio Preto 15054-000, SP, Brazil. anabete@ibilce.unesp.br
Research Domain of This Article
Genetics & Heredity
Article-Type of This Article
Basic Study
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
World J Gastroenterol. Jul 7, 2015; 21(25): 7730-7741 Published online Jul 7, 2015. doi: 10.3748/wjg.v21.i25.7730
TLR2 and TLR4 polymorphisms influence mRNA and protein expression in colorectal cancer
Marcela Alcântara Proença, Juliana Garcia de Oliveira, Aline Cristina Targa Cadamuro, Maysa Succi, João Gomes Netinho, Eny Maria Goloni-Bertolo, Érika Cristina Pavarino, Ana Elizabete Silva
Marcela Alcântara Proença, Aline Cristina Targa Cadamuro, Maysa Succi, Ana Elizabete Silva, Department of Biology, UNESP, São Paulo State University, São José do Rio Preto 15054-000, SP, Brazil
Juliana Garcia de Oliveira, USC- Sacred Heart University, Pró-Reitoria de Pesquisa e Pós Graduação, Bauru 17011-160, SP, Brazil
João Gomes Netinho, Department of Surgery, School of Medicine, FAMERP, São José do Rio Preto 15090-000, SP, Brazil
Eny Maria Goloni-Bertolo, Érika Cristina Pavarino, UPGEM, School of Medicine, FAMERP, São José do Rio Preto 15090-000, SP, Brazil
Author contributions: Proença MA planned and conducted the study, collected and interpreted the data, drafted and wrote the manuscript; de Oliveira JG collected data on genotyping of TLR2 and TLR4 polymorphisms in the control group; Cadamuro ACT collected data on immunohistochemistry of both TLR2 and TLR4 proteins; Succi M collected data on CRC samples; Netinho JG collected data on CRC samples; Goloni-Bertolo EM and Pavarino ÉC planned the study; Silva AE conceived and planned the study and critically revised the manuscript.
Supported by Grants from Brazilian agencies FAPESP, No. 2012/15036-8; and CNPq, No. 304870/2012-9.
Ethics approval: This study was reviewed and approved by the Ethics in Research Committee IBILCE/UNESP, nº027/11 (protocol: 0009.0.229.000-11).
Conflict-of-interest statement: The authors declare no conflicts of interest.
Data sharing statement: All participants gave written informed consent for data sharing.
Correspondence to: Ana Elizabete Silva, PhD, Department of Biology, UNESP, São Paulo State University, Rua Cristóvão Colombo, 2265, São José do Rio Preto 15054-000, SP, Brazil. anabete@ibilce.unesp.br
Telephone: +55-17-32212384 Fax: +55 17-32212390
Received: October 16, 2014 Peer-review started: October 18, 2014 First decision: December 2, 2014 Revised: January 3, 2015 Accepted: February 12, 2015 Article in press: February 13, 2015 Published online: July 7, 2015 Processing time: 264 Days and 14.4 Hours
Core Tip
Core tip: This study investigated the influence of the toll-like receptor (TLR)2 and TLR4 functional polymorphisms on mRNA and protein expression levels in colorectal cancer samples and the association of these polymorphisms with the risk of developing this neoplasm. Increased expression of TLR2 (mRNA and protein) in tumor tissue was observed compared with adjacent normal tissue. Moreover, for the first time, the polymorphism TLR2-196 to -174del was associated with a higher risk of developing this type of cancer, and TLR2-196 to -174del allele carriers showed mRNA relative expression approximately two times higher than wild-genotype carriers. Thus, functional polymorphism in TLR2 may change gene expression levels, accentuating inflammation and aggravating the development of colorectal cancer.
