Isolation and biochemical analysis of vesicles from taurohyodeoxycholic acid-infused isolated perfused rat livers

doi:10.3748/wjg.v19.i37.6228

Advanced Search

BPG is committed to discovery and dissemination of knowledge

Home / Archive / Volume 19, Issue 37

This Article

Peer-Review Report of This Article

Citation of this article

Corresponding Author of This Article

Research Domain of This Article

Article-Type of This Article

Open-Access Policy of This Article

Times Cited Counts in Google of This Article

Number of Hits and Downloads for This Article

Total Article Views (8227)

All Articles published online

The chart showing PDF series, HTML series, Figures (1-3) series, Tables (1-1) series.

Item

Count

PDF

445

HTML

4367

Figures (1-3)

500

Tables (1-1)

512

Sum=5824

Publishing Process of This Article

The chart showing Browse series, Download series.

Item

Count

Browse

1167

Download

1236

Sum=2403

Oct 7, 2013 (publication date) through Mar 23, 2026

Times Cited of This Article

Times Cited (0)

Journal Information of This Article

Publication Name

World Journal of Gastroenterology

ISSN

1007-9327

Publisher of This Article

Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

- Full Article with Cover (PDF)
- Full Article (XML)

Brief Article

World J Gastroenterol. Oct 7, 2013; 19(37): 6228-6236
Published online Oct 7, 2013. doi: 10.3748/wjg.v19.i37.6228

Isolation and biochemical analysis of vesicles from taurohyodeoxycholic acid-infused isolated perfused rat livers

Khalid Rahman, Sahver Ege Hismiogullari, Adnan Adil Hismiogullari

Adnan Adil Hismiogullari, Department of Medical Biochemistry, School of Medicine, The University of Balikesir, 10145 Balikesir, Turkey

Sahver Ege Hismiogullari, Department of Pharmacology and Toxicology, School of Veterinary Medicine, The University of Balikesir, 10145 Balikesir, Turkey

Khalid Rahman, School of Pharmacy and Biomolecular Sciences, Liverpool John Moores University, Liverpool L3 3AF, United Kingdom

Author contributions: All authors contributed equally to the work.

Correspondence to: Dr. Adnan Adil Hismiogullari, Department of Medical Biochemistry, School of Medicine, The University of Balikesir, Cagis Yerleskesi, 10145 Balikesir, Turkey. ahismiogullari@gmail.com

Telephone: +90-26-66121455 Fax: +90-26-66121459

Received: April 19, 2013
Revised: June 12, 2013
Accepted: July 4, 2013
Published online: October 7, 2013
Processing time: 182 Days and 7.6 Hours

Core Tip

Core tip: Bile contains various constituents including cholesterol and phospholipid, mainly phosphatidylcholine with a unique fatty acid composition of 1-palmitoyl 2-linoleyl (16:0-18:2) phosphatidylcholine and 1-palmitoyl 2-oleoyl (16:0-18:1). These biliary lipids are transported in vesicles from a specific intra-hepatic pool and an increase in biliary lipid-carrying vesicles may have implications for hepatic diseases such as gallstone formation. Taurohyodeoxycholic acid (THDC) stimulates the secretion of biliary phospholipids; hence THDC-infused rat livers were subjected to ultracentrifugation in order to isolate these phospholipid-carrying vesicles. The isolation of these biliary lipid-carrying vesicles was not successful; however, vesicles enriched in PDE activity were obtained.