Multiparameter magnetic resonance imaging of liver fibrosis in a bile duct ligation mouse model

doi:10.3748/wjg.v27.i47.8156

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Dec 21, 2021 (publication date) through Mar 1, 2026

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World Journal of Gastroenterology

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World J Gastroenterol. Dec 21, 2021; 27(47): 8156-8165
Published online Dec 21, 2021. doi: 10.3748/wjg.v27.i47.8156

Multiparameter magnetic resonance imaging of liver fibrosis in a bile duct ligation mouse model

Jia-Yi Liu, Zhu-Yuan Ding, Zi-Yi Zhou, Sheng-Zhen Dai, Jie Zhang, Hao Li, Qiu Du, Ye-Yu Cai, Quan-Liang Shang, Yong-Heng Luo, En-Hua Xiao

Jia-Yi Liu, Zhu-Yuan Ding, Zi-Yi Zhou, Sheng-Zhen Dai, Jie Zhang, Ye-Yu Cai, Quan-Liang Shang, Yong-Heng Luo, En-Hua Xiao, Department of Radiology, The Second Xiangya Hospital, Central South University, Changsha 410011, Hunan Province, China

Hao Li, Department of Emergency Medicine, First People's Hospital of Changde City, Changde 415000, Hunan Province, China

Qiu Du, Department of Urology, Hunan Provincial People's Hospital, First Affiliated Hospital of Hunan Normal University, Changsha 410005, Hunan Province, China

Author contributions: Liu JY, Luo YH and Xiao EH designed and coordinated the study; Liu JY, Ding ZY, Dai SZ performed the experiments, Li H, Du Q, Cai YY and Shang QL acquired and analyzed data; Shang QL interpreted the data; Liu JY and Luo YH wrote the manuscript; Luo YH and Xiao EH jointly supervised this work; all authors approved the final version of the article.

Supported by the Natural Science Foundation of Hunan Province, No. 2019JJ40444 (to Xiao EH) and 2021JJ30945 (to Luo YH).

Institutional review board statement: The protocol of our study was approved by the Animal Care and Use Committee of the Second Xiangya Hospital of Central South University (Approval No. 2020495).

Conflict-of-interest statement: None of the authors have identified a conflict of interest.

Data sharing statement: No additional data are available.

Corresponding author: Yong-Heng Luo, PhD, Attending Doctor, Department of Radiology, The Second Xiangya Hospital, Central South University, No. 139 Renming Middle Road, Changsha 410011, Hunan Province, China. luoyongheng@csu.edu.cn

Received: April 18, 2021
Peer-review started: April 18, 2021
First decision: June 13, 2021
Revised: June 15, 2021
Accepted: September 19, 2021
Article in press: September 19, 2021
Published online: December 21, 2021
Processing time: 242 Days and 20.3 Hours

ARTICLE HIGHLIGHTS

Research background

Bile duct ligation (BDL) is a classical method for mimicking cholestatic fibrosis in animals. Magnetic resonance imaging (MRI) has enabled significant advances in noninvasive assessment of liver fibrosis. More comprehensive liver fibrotic features of BDL on MRI are important. However, the utility of multiparameter MRI to detect liver fibrosis in a BDL mouse model has not been assessed.

Research motivation

We hypothesized that the BDL mouse model can be characterized radiologically using MRI, which is a noninvasive method. The model can be further validated regarding radiological, quantitative, and analytical aspects.

Research objectives

Using a BDL mouse model to evaluate the correlation between the pathological changes and several parameters of MRI characteristics of liver fibrosis.

Research methods

Twenty-eight healthy adult male balb/c mice were included. They were randomly divided into four groups: Sham group and week 2 BDL, week 4 BDL and week 6 BDL groups. The MRI sequences included the following parameters: Magnetic resonance cholangiopancreatography (MRCP), T1-weighted, T2-weighted, T2 mapping, and pre- and post-enhanced T1 mapping. All these were performed after sham and BDL surgery. Peripheral blood and liver tissue were collected after the MRI.

Research results

The bile duct volume, ΔT1 value, T2 value, and the rate of liver fibrosis increased steadily in the week 2 BDL, week 4 BDL and week 6 BDL groups compared to those in the sham group (P < 0.01). Strong correlations were found between bile duct volume, T2 value, ΔT1 value, and hepatic fibrosis rate (all P < 0.01) in all BDL groups.

Research conclusions

The BDL mouse model induces changes that are easily observed using MRI. The MRI parameters correlate with the hepatic fibrosis rate and enable the detection of cholestatic fibrosis.

Research perspectives

We believe that advanced MR techniques have considerable potential for widespread application in preclinical and clinical fields.