Colorectal Cancer
Copyright ©The Author(s) 2003. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Aug 15, 2003; 9(8): 1713-1718
Published online Aug 15, 2003. doi: 10.3748/wjg.v9.i8.1713
Refinement of heterozygosity loss on chromosome 5p15 in sporadic colorectal cancer
Shi-Feng Xu, Zhi-Hai Peng, Da-Peng Li, Guo-Qiang Qiu, Fang Zhang
Shi-Feng Xu, Zhi-Hai Peng, Da-Peng Li, Guo-Qiang Qiu, Fang Zhang, Department of General Surgery, Shanghai First People’s Hospital, Shanghai 200080, China
Author contributions: All authors contributed equally to the work.
Supported by the National Natural Science Foundation of China, No. 30080016
Correspondence to: Dr. Zhi-Hai Peng, Department of General Surgery, Shanghai First People’s Hospital, 85 Wujin Road, Shanghai 200080, China. pengpzhb@online.sh.cn
Telephone: +86-21-63240090 Ext 3102
Received: March 20, 2003
Revised: April 2, 2003
Accepted: April 17, 2003
Published online: August 15, 2003
Abstract

AIM: To refine the loss of heterozygosity on chromosome 5p15 and to identify the new tumor suppressor gene (s) in colorectal tumorigenesis.

METHODS: Sixteen polymorphic microsatellite markers were analyzed on chromosome 5 and another 6 markers were applied on chromosome 5p15 in 83 cases of colorectal and normal DNA by PCR. PCR products were electrophoresed on an ABI 377 DNA sequencer. Genescan 3.1 and Genotype 2.1 software were used for LOH scanning and analysis.

RESULTS: We observed 2 distinct regions of frequent allelic deletions on Chromosome 5, at D5S416 on 5p15 and D5S428-D5S410 on 5q. Another 6 polymorphric microsatellite markers were applied to 5p15 and the minimal region of frequent loss of heterozygosity was established on 5p15 spanning the D5S416 locus.

CONCLUSION: Through our detailed deletion mapping studies, we have found a critical and precise location of 5p deletions, 5p15.2-5p15.3, which must contain one or more unknown tumor suppressor gene (s) of colorectal cancer.

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