Brief Reports
Copyright ©The Author(s) 2003. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Jul 15, 2003; 9(7): 1594-1597
Published online Jul 15, 2003. doi: 10.3748/wjg.v9.i7.1594
Effects of carbon dioxide and nitrogen on adhesive growth and expressions of E-cadherin and VEGF of human colon cancer cell CCL-228
Kai-Lin Cai, Guo-Bing Wang, Li-Juan Xiong
Kai-Lin Cai, Guo-Bing Wang, General Surgery Department, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China
Li-Juan Xiong, Infectious Disease Department, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China
Author contributions: All authors contributed equally to the work.
Supported by the Natural Science Foundation of Hubei Province, No. 2000J062
Correspondence to: Dr. Kai-Lin Cai, General Surgery Department, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022,Hubei Province, China. caikailin@yahoo.com.cn
Telephone: +86-27-85726405 Fax: +86-27-85776343
Received: March 3, 2003
Revised: March 24, 2003
Accepted: April 5, 2003
Published online: July 15, 2003
Abstract

AIM: To study the effects of carbon dioxide on the metastatic capability of cancer cells, and to compare them with that of nitrogen.

METHODS: The colon cancer cell CCL-228 was treated with 100% carbon dioxide or nitrogen at different time points and then cultured under normal condition. Twelve hours after the treatment, the survival rates of suspension cells and the expressions of e-cadherin and VEGF were examined.

RESULTS: After 60 min of carbon dioxide and longer time of nitrogen treatment, the suspended cells increased and the expression of e-cadherin decreased while the expression of VEGF was enhanced significantly. And the effects of nitrogen were similar to, but weaker than, those of carbon dioxide.

CONCLUSION: Carbon dioxide may improve the metastatic capability of cancer cells and its effects are significantly stronger than that of nitrogen. A sequential use of carbon dioxide and nitrogen in pneumoperitoneum may take the advantage of both gases.

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