Cai KL, Wang GB, Xiong LJ. Effects of carbon dioxide and nitrogen on adhesive growth and expressions of E-cadherin and VEGF of human colon cancer cell CCL-228. World J Gastroenterol 2003; 9(7): 1594-1597 [PMID: 12854171 DOI: 10.3748/wjg.v9.i7.1594]
Corresponding Author of This Article
Dr. Kai-Lin Cai, General Surgery Department, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022,Hubei Province, China. caikailin@yahoo.com.cn
Article-Type of This Article
Brief Reports
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA
Share the Article
Cai KL, Wang GB, Xiong LJ. Effects of carbon dioxide and nitrogen on adhesive growth and expressions of E-cadherin and VEGF of human colon cancer cell CCL-228. World J Gastroenterol 2003; 9(7): 1594-1597 [PMID: 12854171 DOI: 10.3748/wjg.v9.i7.1594]
World J Gastroenterol. Jul 15, 2003; 9(7): 1594-1597 Published online Jul 15, 2003. doi: 10.3748/wjg.v9.i7.1594
Effects of carbon dioxide and nitrogen on adhesive growth and expressions of E-cadherin and VEGF of human colon cancer cell CCL-228
Kai-Lin Cai, Guo-Bing Wang, Li-Juan Xiong
Kai-Lin Cai, Guo-Bing Wang, General Surgery Department, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China
Li-Juan Xiong, Infectious Disease Department, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China
Author contributions: All authors contributed equally to the work.
Supported by the Natural Science Foundation of Hubei Province, No. 2000J062
Correspondence to: Dr. Kai-Lin Cai, General Surgery Department, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022,Hubei Province, China. caikailin@yahoo.com.cn
Telephone: +86-27-85726405 Fax: +86-27-85776343
Received: March 3, 2003 Revised: March 24, 2003 Accepted: April 5, 2003 Published online: July 15, 2003
Abstract
AIM: To study the effects of carbon dioxide on the metastatic capability of cancer cells, and to compare them with that of nitrogen.
METHODS: The colon cancer cell CCL-228 was treated with 100% carbon dioxide or nitrogen at different time points and then cultured under normal condition. Twelve hours after the treatment, the survival rates of suspension cells and the expressions of e-cadherin and VEGF were examined.
RESULTS: After 60 min of carbon dioxide and longer time of nitrogen treatment, the suspended cells increased and the expression of e-cadherin decreased while the expression of VEGF was enhanced significantly. And the effects of nitrogen were similar to, but weaker than, those of carbon dioxide.
CONCLUSION: Carbon dioxide may improve the metastatic capability of cancer cells and its effects are significantly stronger than that of nitrogen. A sequential use of carbon dioxide and nitrogen in pneumoperitoneum may take the advantage of both gases.
