Effect of compound rhodiola sachalinensis A Bor on CCl4-induced liver fibrosis in rats and its probable molecular mechanisms

doi:10.3748/wjg.v9.i7.1559

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Jul 15, 2003 (publication date) through Nov 27, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Basic Research

World J Gastroenterol. Jul 15, 2003; 9(7): 1559-1562
Published online Jul 15, 2003. doi: 10.3748/wjg.v9.i7.1559

Effect of compound rhodiola sachalinensis A Bor on CCl₄-induced liver fibrosis in rats and its probable molecular mechanisms

Xiao-Ling Wu, Wei-Zheng Zeng, Pi-Long Wang, Chun-Tao Lei, Ming-De Jiang, Xiao-Bin Chen, Yong Zhang, Hui Xu, Zhao Wang

Xiao-Ling Wu, Pi-Long Wang, Chun-Tao Lei, Department of Gastroenterology, First Affiliated Hospital, Chongqing University of Medical Sciences, Chongqing 400016, China

Wei-Zheng Zeng, Ming-De Jiang, Xiao-Bin Chen, Yong Zhang, Hui Xu, Zhao Wang, Department of Digestion, General Hospital of Chengdu Military Command, Chengdu 610083, Sichuan Province, China Supported by the Tenth-Five Year Plan of Medical Science Foundation of Chengdu Military Command, No. 01A009

Author contributions: All authors contributed equally to the work.

Correspondence to: Professor Wei-Zheng Zeng, Department of Digestion, General Hospital of Chengdu Military Command, Chengdu 610083, Sichuan Province, China. zengweizheng@163.com

Telephone: +86-28-86570347

Received: November 6, 2002
Revised: December 4, 2002
Accepted: December 30, 2002
Published online: July 15, 2003

Abstract

AIM: To explore the anti-fibrotic effect of a traditional Chinese medicine, compound rhodiola sachalinensis A Bor on CCl₄-induced liver fibrosis in rats and its probable molecular mechanisms.

METHODS: Ninety healthy male SD rats were randomly divided into three groups: normal group (n = 10), treatment group of compound rhodiola sachalinensis A Bor (n = 40) and CCl₄-induced model group (n = 40). The liver fibrosis was induced by CCl₄ subcutaneous injection. Treatment group was administered with compound rhodiola sachalinensis A Bor (0.5 g/kg) once a day at the same time. Then the activities of several serum fibrosis-associated enzymes: alanine aminotransferase (ALT), aspartate aminotransferase (AST), N-acetyl-beta-D-glucosaminidase (β-NAG) and the levels of serum procollagen III (PC III), collagen IV (C IV), hyaluronic acid (HA) were assayed. The histopathological changes were observed with HE, VG and Masson stain. The expression of TGF-β1 mRNA, α1 (I) mRNA and Na⁺/Ca²⁺ exchanger (NCX) mRNA was detected by reverse transcription polymerase chain reaction (RT-PCR) in situ.

RESULTS: Compound rhodiola sachalinensis A Bor significantly reduced serum activities of ALT, AST, β-NAG and decreased the levels of PC III, C IV, HA, improved the liver histopathological changes, inhibited the expression of TGF-β1 mRNA, α(I) mRNA and Na⁺/Ca²⁺ exchanger mRNA in rats.

CONCLUSION: Compound rhodiola sachalinensis A Bor can intervene in CCl₄-induced liver fibrosis in rats, in which potential mechanisms may be decreasing the production of TGF-β1, reducing the production of collagen, preventing the activation of hepatic stellate cell (HSC) and inhibiting the expression of TGF-β1 mRNA, α1(I) mRNA and Na⁺/Ca²⁺ exchanger mRNA.

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