Clinical Research
Copyright ©The Author(s) 2003. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Apr 15, 2003; 9(4): 851-857
Published online Apr 15, 2003. doi: 10.3748/wjg.v9.i4.851
Ultrastructure and molecular biological changes of chronic gastritis, gastric cancer and gastric precancerous lesions: a comparative study
Goang-Yao Yin, Wu-Ning Zhang, Xiao-Jing Shen, Yi Chen, Xue-Fen He
Goang-Yao Yin, Xiao-Jing Shen, Xue-Fen He, Wuxi No.3 Peoples Hospital, Wuxi 214041, Jiangsu Province, China
Wu-Ning Zhang, Yi Chen, Department of National Microanalysis Center, Fudan University, Shanghai 200433, Shanghai, China
Author contributions: All authors contributed equally to the work.
Correspondence to: Dr. Goang-Yao Yin, Wuxi No.3 Peoples Hospital, 230 Eastern Tonghhui Road Wuxi 214041, Jiangsu Province,China. yinyao@pub.wx.jsinfo.net
Received: October 4, 2002
Revised: November 26, 2002
Accepted: December 3, 2002
Published online: April 15, 2003
Abstract

AIM: To carry out a comparative study on ultrastructure and molecular biological changes of chronic gastritis (CG), gastric cancer (GC) aand gastric precancerous lesions.

METHODS: By the use of histochemical staining, SEM with EDAX, TEM with EDAX, image analysis technique, RIA and chemiluminescence method, gastric mucosa of 168 patients were synchronously analyzed in morphology, trace elements, DNA, cAMP, SOD, 3H-TdR LCT and serum LPO were also done.

RESULTS: The incidence of epithelial nucleoplasmic ratio > 1, lobulated nuclei, inter-chromatin aggregation of granules, nucleolar hypertrophy, and the content of DNA, Zn, Cu in nuclei and serum LPO of each group were showed as belows: normal control group (0.0, 0.0, 6.7, 0.0, 12.6 ± 2.7, 7.6 ± 0.4, 58.4 ± 0.3, 2.6 ± 0.6), CSG group (5.7, 2.9, 7.4, 2.9, 15.2 ± 3.1, 8.1 ± 0.5, 58.9 ± 0.5, 4.2 ± 0.7), CAG group (31.3, 29.7, 45.3, 42.2, 16.5 ± 3.1, 8.6 ± 0.4, 59.3 ± 0.5, 4.5 ± 0.6), CA group (100.0, 100.0, 72.2, 50.0, 30.7 ± 8.2, 8.8 ± 0.3, 59.5 ± 0.4, 6.8 ± 1.6), ATP++ group (61.5, 38.5, 23.1, 38.5, 23.5 ± 8.9, 8.3 ± 0.4, 59.1 ± 0.4, 5.1 ± 1.2), IM++ + ATP++ group (77.8, 55.5, 33.3, 44.4, 25.1 ± 7.2, 8.4 ± 0.5, 59.5 ± 0.4, 6.5 ± 1.1), IM+++ + ATP++ group (100.0, 100.0, 75.0, 62.5, 28.5 ± 9.1, 8.9 ± 0.5, 59.7 ± 0.4, 7.6 ± 0.7), IMIIb group (100.0, 62.5, 75.0, 50.0, 27.3 ± 10.3, 8.6 ± 0.3, 59.5 ± 0.4, 6.1 ± 0.9); whereas the content of Zn, Cu in mitochondria and cAMP, SOD in gastric mucosa, and 3H-TdR LCT of each group were showen as belows: normal control group (9.2 ± 0.5, 58.3 ± 0.3, 15.9 ± 1.5, 170.5 ± 6.1, 1079.7 ± 227.4), CSG group (8.6 ± 0.5, 57.8 ± 0.3, 14.6 ± 1.8, 163.3 ± 5.6, 867.3 ± 240.5), CAG group (8.3 ± 0.4, 57.5 ± 0.3, 13.4 ± 1.8, 161.2 ± 4.3, 800.9 ± 221.8), CA group (8.9 ± 0.4, 57.1 ± 0.3, 10.2 ± 3.9, 152.2 ± 3.8, 325.7 ± 186.8), ATP++ group (9.1 ± 0.4, 57.0 ± 0.3, 12.4 ± 1.8, 161.5 ± 3.8, 642.9 ± 174.3), IM++ + ATP++ group (8.6 ± 0.4, 56.9 ± 0.3, 12.0 ± 2.3, 152.2 ± 2.5, 326.3 ± 160.3), IM+++ + ATP++ group (8.5 ± 0.3, 56.8 ± 0.2, 10.4 ± 0.9, 147.4 ± 2.6, 316.1 ± 170.7), IMIIb group (8.6 ± 0.3, 56.9 ± 0.3, 11.9 ± 1.9, 150.0 ± 2.8, 318.9 ± 145.8), there were significant differences between groups (P < 0.05-0.01).

CONCLUSION: There was a significant difference between CG and GC in their ultrastructure and molecular biology. Only on the condition of changes of internal environment in combination with the harmful effect of external environment, chronic atrophic gastritis can then develop into gastric cancer. Hence it might have similar epithelial cell ultrastructure and molecular biological changes in ATP++, IMIIb and cancer, hence there were similar patterns of occurrence, development and transformation. Recognition of this trend might help to explore problems of prevention and cure.

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