Viral Hepatitis
Copyright ©The Author(s) 2003. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Apr 15, 2003; 9(4): 751-754
Published online Apr 15, 2003. doi: 10.3748/wjg.v9.i4.751
Effect of alpha 2b interferon on inducement of mIL-2R and treatment of HCV in PBMC from patients with chronic viral hepatitis C
Jian Wang, Gui-Ju Xiang, Bing-Xiang Liu
Jian Wang, Department of Aetiology and Immunology, Anhui University of Science and Technology, Huainan 232001, Anhui Province, China
Gui-Ju Xiang, Department of Infectious Diseases, the Second Miner Hospital of Huainan, Huainan 232001, Anhui Province, China
Bing-Xiang Liu, Department of Infectious Diseases, Shibei Hospital of Shanghai, Shanghai 200435, China
Author contributions: All authors contributed equally to the work.
Supported by the grants from Science Foundation of the Ministry of Coal Industry of China, No. 96-072
Correspondence to: Jian Wang, Department of Aetiology and Immunology, Anhui University of Science and Technology, Huainan 232001, Anhui Province, China. wangjian8237@sina.com
Telephone: +86-0554-6659942
Received: July 31, 2002
Revised: August 16, 2002
Accepted: August 23, 2002
Published online: April 15, 2003
Abstract

AIM: To study the level of membrane interleukin-2 receptor (mIL-2R) on surface of peripheral blood mononuclear cells (PBMC) and the therapeutic efficacy of alpha 2b interferon on the treatment of HCV-RNA in PBMC of patients with chronic hepatitis C and to compare the negative rates of HCV-RNA in PBMC, HCV-RNA and anti-HCV in serum.

METHODS: Before and after treatment of alpha 2b interferon, the level of mIL-2R of patients with chronic hepatitis C was detected by biotin-streptavidin (BSA). The therapeutic group (26 cases) was treated with alpha 2b interferon (3 MU/d) and control therapeutic group (22 cases) was treated with routine drugs (VitC, aspartic acid). The total course of treatment with alpha 2b interferon and routine drug was six months and per course of the treatment was three months. The levels of HCV-RNA in PBMC, HCV-RNA and anti-HCV in serum were detected before and after a course of the treatment.

RESULTS: Before and after treatment of alpha 2b interferon and routine drugs, the levels of mIL-2R in silence stage were (3.44 ± 0.77)% and (2.95 ± 0.72)%, the levels of mIL-2R in inducement stage were (33.62 ± 3.95)% and (30.04 ± 3.73)%. There was a significant difference between two groups (P < 0.01-P < 0.05). After treatment of alpha 2b interferon with 3 MU/d for two courses of the treatment, the total negative rates of HCV-RNA in the PBMC and HCV-RNA, anti-HCV in serum were 42.31% (11/26), 57.69% (15/26), 65.38%(17/26) respectively. After the treatment of routine drug, the negative rates of HCV-RNA in PBMC and HCV-RNA, anti-HCV in serum were 13.64% (3/22), 22.73% (5/22), 27.27% (6/22) respectively. There was high significant difference in the group treated with alpha 2b interferon and the group treated with routine drugs (P < 0.01-P < 0.05).

CONCLUSION: The mIL-2R can be induced by alpha 2b interferon during the treatment. The alpha 2b interferon has a definite effect on the treatment of HCV-RNA in PBMC. The curative effect of alpha 2b interferon is better than that of the routine drugs.

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