Docetaxel inhibits SMMC-7721 human hepatocellular carcinoma cells growth and induces apoptosis

doi:10.3748/wjg.v9.i4.696

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Apr 15, 2003 (publication date) through Nov 26, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Liver Cancer

World J Gastroenterol. Apr 15, 2003; 9(4): 696-700
Published online Apr 15, 2003. doi: 10.3748/wjg.v9.i4.696

Docetaxel inhibits SMMC-7721 human hepatocellular carcinoma cells growth and induces apoptosis

Chang-Xin Geng, Zhao-Chong Zeng, Ji-Yao Wang

Chang-Xin Geng, Ji-Yao Wang, Department of Gastroenterology, Zhongshan Hospital, Fudan University, Shanghai 200032, China

Zhao-Chong Zeng, Department of Radiation Oncology, Zhongshan Hospital, Fudan University, Shanghai 200032, China

Author contributions: All authors contributed equally to the work.

Correspondence to: Prof. Ji-Yao Wang, Director of Department of Gastroenterology, Zhongshan Hospital, Fudan University, Shanghai 200032, China. jiyao_wang@hotmail.com

Telephone: +86-21-64041990-2420 Fax: +86-21-34160980

Received: August 6, 2002
Revised: August 26, 2002
Accepted: September 3, 2002
Published online: April 15, 2003

Abstract

AIM: To investigate the in vitro anti-hepatocellular carcinoma (HCC) activity of docetaxel against SMMC-7721 HCC cells and its possible mechanism.

METHODS: The HCC cells were given different concentrations of docetaxel and their growth was measured by colony forming assay. Cell cycle and apoptosis were analyzed by flow cytometry and fluorescence microscopy (acridine orange/ethidium bromide double staining, AO/EB), as well as electronic microscopy. The SMMC-7721 HCC cell reactive oxygen species (ROS) and glutathione (GSH) were measured after given docetaxel.

RESULTS: Docetaxel inhibited the hepatocellular carcinoma cells growth in a concentration dependent manner with IC₅₀ 5 × 10^-10 M. Marked cell apoptosis and G2/M phase arrest were observed after treatment with docetaxel ≥ 10^-8 M. Docetaxel promoted SMMC-7721 HCC cells ROS generation and GSH deletion.

CONCLUSION: Docetaxel suppressed the growth of SMMC-7721 HCC cells in vitro by causing apoptosis and G2/M phase arrest of the human hepatoma cells, and ROS and GSH may play a key role in the inhibition of growth and induction of apoptosis.

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