Li QF, Ouyang GL, Peng XX, Hong SG. Effects of tachyplesin on the regulation of cell cycle in human hepatocarcinoma SMMC-7721 cells. World J Gastroenterol 2003; 9(3): 454-458 [PMID: 12632496 DOI: 10.3748/wjg.v9.i3.454]
Corresponding Author of This Article
Dr. Qi-Fu Li, The Key Laboratory of China Education Ministry for Cell Biology and Tumor Cell Engineering, School of Life Sciences, Xiamen University, Xiamen 361005, Fujian Province, China. chifulee@163.net
Article-Type of This Article
Liver Cancer
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World J Gastroenterol. Mar 15, 2003; 9(3): 454-458 Published online Mar 15, 2003. doi: 10.3748/wjg.v9.i3.454
Effects of tachyplesin on the regulation of cell cycle in human hepatocarcinoma SMMC-7721 cells
Qi-Fu Li, Gao-Liang Ouyang, Xuan-Xian Peng, Shui-Gen Hong
Qi-Fu Li, Xuan-Xian Peng, The Key Laboratory of China Education Ministry for Cell Biology and Tumor Cell Engineering, School of Life Sciences, Xiamen University, Xiamen 361005, Fujian Province, China
Gao-Liang Ouyang, Shui-Gen Hong, Laboratory of Cell Biology, School of Life Sciences, Xiamen University, Xiamen 361005, Fujian Province, China
Author contributions: All authors contributed equally to the work.
Supported by the National Natural Science Foundation of China, No. 30170724
Correspondence to: Dr. Qi-Fu Li, The Key Laboratory of China Education Ministry for Cell Biology and Tumor Cell Engineering, School of Life Sciences, Xiamen University, Xiamen 361005, Fujian Province, China. chifulee@163.net
Telephone: +86-592-2183619 Fax: +86-592-2186392
Received: August 26, 2002 Revised: September 23, 2002 Accepted: October 29, 2002 Published online: March 15, 2003
Abstract
AIM: To investigate the effects of tachyplesin on the cell cycle regulation in human hepatcarcinoma cells.
METHODS: Effects of tachyplesin on the cell cycle in human hepatocarcinoma SMMC-7721 cells were assayed with flow cytometry. The protein levels of p53, p16, cyclin D1 and CDK4 were assayed by immunocytochemistry. The mRNA levels of p21WAF1/CIP1 and c-myc genes were examined with in situ hybridization assay.
RESULTS: After tachyplesin treatment, the cell cycle arrested at G0/G1 phase, the protein levels of mutant p53, cyclin D1 and CDK4 and the mRNA level of c-myc gene were decreased, whereas the levels of p16 protein and p21WAF1/CIP1 mRNA increased.
CONCLUSION: Tachyplesin might arrest the cell at G0/G1 phase by upregulating the levels of p16 protein and p21WAF1/CIP1 mRNA and downregulating the levels of mutant p53, cyclin D1 and CDK4 proteins and c-myc mRNA, and induce the differentiation of human hepatocacinoma cells.
