Basic Research
Copyright ©The Author(s) 2003. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Dec 15, 2003; 9(12): 2782-2785
Published online Dec 15, 2003. doi: 10.3748/wjg.v9.i12.2782
Changes of biological functions of dipeptide transporter (PepT1) and hormonal regulation in severe scald rats
Bing-Wei Sun, Xiao-Chen Zhao, Guang-Ji Wang, Ning Li, Jie-Shou Li
Bing-Wei Sun, Ning Li, Jie-Shou Li, Department of General Surgery, School of Medicine, Nanjing University, Nanjing 210093, Jiangsu Province, China
Bing-Wei Sun, Ning Li, Jie-Shou Li, Research Institute of General Hospital, Chinese PLA General Hospital of Nanjing Military Command, Nanjing 210002, Jiangsu Province, China
Xiao-Chen Zhao, Guang-Ji Wang, Center of Drug Metabolism and Pharmacokinetics, China Pharmaceutical University, Nanjing 210009, Jiangsu Province, China
Author contributions: All authors contributed equally to the work.
Supported by National Natural Science Foundation of China, No. 39970862
Correspondence to: Bing-Wei Sun, Research Institute of General Surgery, Chinese PLA General Hospital of Nanjing Military Command, 305 East Zhongshan Road, Nanjing 210002, Jiangsu Province, China. sunbinwe@hotmail.com
Telephone: +86-25-3387871 Ext 58088 Fax: +86-25-4803956
Received: June 10, 2003
Revised: June 22, 2003
Accepted: August 16, 2003
Published online: December 15, 2003
Abstract

AIM: To determine the regulatory effects of recombinant human growth hormone (rhGH) on dipeptide transport (PepT1) in normal and severe scald rats.

METHODS: Male Sprague-Dawley rats with 30% total body surface area (TBSA)IIIdegree scald were employed as the model. In this study rhGH was used at the dose of 2 IU.kg-1d-1. An everted sleeve of intestine 4 cm long obtained from mid-jejunum was securely incubated in Kreb’s solution with radioactive dipeptide (3H-glycylsarcosine, 3H-Gly-Sar, 10 μCi/ml) at 37 °C for 15 min to measure the effects of uptake and transport of PepT1 of small intestinal epithelial cells in normal and severe scald rats.

RESULTS: Abundant blood supply to intestine and mesentery was observed in normal and scald rats administered rhGH, while less supply of blood to intestine and mesentery was observed in rats without rhGH. Compared with controls, the transport of dipeptide in normal rats with injection of rhGH was not significantly increased (P = 0.1926), while the uptake was significantly increased (P = 0.0253). The effects of transport and uptake of PepT1 in scald rats with injection of rhGH were significantly increased (P = 0.0082, 0.0391).

CONCLUSION: Blood supply to intestine and mesentery of rats was increased following injection of rhGH. The effects of uptake and transport of dipeptide transporters in small intestinal epithelial cells of rats with severe scald were markedly up-regulated by rhGH.

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