Inhibitory effects of antisense RNA of HAb18G/CD147 on invasion of hepatocellular carcinoma cells in vitro

doi:10.3748/wjg.v9.i10.2174

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Oct 15, 2003 (publication date) through Nov 8, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

Liver Cancer

World J Gastroenterol. Oct 15, 2003; 9(10): 2174-2177
Published online Oct 15, 2003. doi: 10.3748/wjg.v9.i10.2174

Inhibitory effects of antisense RNA of HAb18G/CD147 on invasion of hepatocellular carcinoma cells in vitro

Yu Li, Peng Shang, Ai-Rong Qian, Li Wang, Yong Yang, Zhi-Nan Chen

Yu Li, Peng Shang, Ai-Rong Qian, Li Wang, Yong Yang, Zhi-Nan Chen, Department of Cell Biology, Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China

Author contributions: All authors contributed equally to the work.

Supported by National Natural Science Foundation of China, No. 39989002

Correspondence to: Dr. Zhi-Nan Chen, Department of Cell Biology, Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China. chcerc2 @fmmu.edu.cn

Telephone: +86-29-3374547 Fax: +86-29-3293906

Received: May 11, 2003
Revised: May 28, 2003
Accepted: June 7, 2003
Published online: October 15, 2003

Abstract

AIM: To study the inhibitory effects of antisense RNA of HAb18G/CD147 on invasion of hepatocellular carcinoma (HCC) cells in vitro.

METHODS: Antisense RNA of HAb18G/CD147 vector PCI-asHAb18G was constructed by reversely inserting HAb18G/CD147 cDNA to eukaryotic expression vector PCI-neo. The HCC cell line HHCC was transfected by PCI-asHAb18G via cation liposome. Expression of HAb18G/CD147 of transfected cells selected by G418 (geneticin) was observed by immuno-histochemical SP staining and FACS (fluorescence activated cell sorting). Gelatin zymography was used to determine the effect of PCI-asHAb18G on reducing secretions of MMP-2 and MMP-9 of the transfected cells. Boyden chamber was employed to test the invasion of HCC cells in vitro.

RESULTS: The construction of antisense RNA vector PCI-asHAb18G was verified correct by partial nucleotide sequencing and restricted endonuclease digestion. The expression of HAb18G/CD147 in transfected HHCC was inhibited by PCI-asHAb18G. Secretions of MMP-2 and MMP-9 of transfected HHCC were reduced and the invasion of transfected HHCC was inhibited compared to HHCC, respectively.

CONCLUSION: Invasion of HCC cells can be inhibited by antisense RNA of HAb18G/CD147. HAb18G/CD147 may be used as a potential target of drugs for anti-invasion and metastasis of HCC.

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