Basic Research
Copyright ©The Author(s) 2002. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Apr 15, 2002; 8(2): 375-378
Published online Apr 15, 2002. doi: 10.3748/wjg.v8.i2.375
Gastrin, somatostatin, G and D cells of gastric ulcer in rats
Feng-Peng Sun, Yu-Gang Song, Wei Cheng, Tong Zhao, Yong-Li Yao
Feng-Peng Sun, Yu-Gang Song, Wei Cheng, Tong Zhao, Yong-Li Yao, Department of Gastroenterology, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, Guangdong Province, China
Author contributions: All authors contributed equally to the work.
Supported by Natural Science Foundation of Guangdong Province, China, No.010578, and Important Technological Issue of Guangdong Province, No.99-13
Correspondence to: Feng-Peng Sun, Department of Gastroenterology, Zhujiang Hospital, First Military Medical University, Guangzhou 510282, Guangdong Province, China. sci@china.com
Telephone: +86-20-85140114-87101
Received: May 30, 2001
Revised: November 1, 2001
Accepted: November 10, 2001
Published online: April 15, 2002
Abstract

AIM: To investigate the relationship among gastrin, somatostatin, G and D cells in gastric ulcer and in its healing process in rats.

METHODS: Fourty-nine Wistar rats were divided into 7 groups. The gastric ulcer model was induced by acetic acid successfully. The gastrin and the somatostatin in rat plasma, gastric fluid and antral tissue were measured by radioimmunoassay (RIA). G and D cells in antral mucosa were analyzed with polyclonal antibody of gastrin and somatostatin by immunohistochemical method and Quantimet 500 image analysis system.

RESULTS: In gastric ulcer, the level of gastrin in plasma, gastric fluid, and antral tissue increased, that of somatostatin declined, and the disorder gradually recovered to the normal level in the healing process. Immunohistochemical technique of G and D cells in antral mucosa demonstrated that the number of G cells increased and that of D cells decreased, both areas of G and D cells declined, the ratio of number and area of G/D increased in gastric ulcer, and the disorder gradually recovered in the healing process.

CONCLUSION: In gastric ulcer, the increased gastrin secreted by G cells, the declined somatostatin secreted by D cells, and the disordered G/D cell ratio can lead to gastrointestinal dysfunction.

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