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Ren B, Zhu HQ, Luo ZF, Zhou Q, Wang Y, Wang YZ. Preliminary research on myosin light chain kinase in rabbit liver. World J Gastroenterol 2001; 7(6): 868-871 [PMID: 11854919 DOI: 10.3748/wjg.v7.i6.868]
World J Gastroenterol. Dec 15, 2001; 7(6): 868-871 Published online Dec 15, 2001. doi: 10.3748/wjg.v7.i6.868
Preliminary research on myosin light chain kinase in rabbit liver
Bin Ren, Hua-Qing Zhu, Zhao-Feng Luo, Qing Zhou, Yuan Wang, Yu-Zhen Wang
Bin Ren, Zhao-Feng Luo, Yu-Zhen Wang, Department of Biochemistry and Molecular Biology, University of Science and Technology of China, Hefei 230027, Anhui Province, China
Hua-Qing Zhu, Qing Zhou, Yuan Wang, Laboratory of Molecular Biology, Anhui Medical University, Hefei 230032, Anhui Province, China
Author contributions: All authors contributed equally to the work.
Supported by the National Natural Science Foundation of China (39870324, YW), Key Innovation Project of Chinese Academy of Science, P.R. China (KSCX 2-2-01, XB Yao), Grant for Excellent Young Teachers of Ministry of Education of China (99044312, YW) & Natural Science Foundation of Anhui Province (99044312, YW).
Correspondence to: Prof. Yu-Zhen Wang, School of Life Science, University of Science and Technology of China, Hefei 230027, Anhui Province, China. yzwang@ustc.edu.cn
Telephone: +86-551-5118963, Fax: +86-551-3601441
Received: July 3, 2001 Revised: July 9, 2001 Accepted: July 16, 2001 Published online: December 15, 2001
Abstract
AIM: To study preliminarily the properties of myosin light chain kinase (MLCK) in rabbit liver.
METHODS: The expression of MLCK was detected by reverse transcription-polymerase chain reaction (RT-PCR); the MLCK was obtained from rabbit liver, and its activity was analyzed by γ-32 P incorporation technique to detect the phosphorylation of myosin light chain.
RESULTS: MLCK was expressed in rabbit liver, and the activity of the enzyme was similar to rabbit smooth muscle MLCK, and calmodulin- dependent. When the concentration was 0.65 mg •L¯¹, the activity was at the highest level.
CONCLUSION: MLCK expressed in rabbit liver may catalyze the phosphorylation of myosin light chain, which may play important roles in the regulation of hepatic cell functions.
