Polymorphism of flagellin A gene in Helicobacter pylori

doi:10.3748/wjg.v7.i6.783

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Dec 15, 2001; 7(6): 783-787
Published online Dec 15, 2001. doi: 10.3748/wjg.v7.i6.783

Polymorphism of flagellin A gene in Helicobacter pylori

Wan-Sheng Ji, Jia-Lu Hu, Jun-Wen Qiu, Dao-Rong Peng, Bing-Long Shi, Shao-Juan Zhou, Kai-Chun Wu, Dai-Ming Fan

Wan-Sheng Ji, Jia-Lu Hu, Jun-Wen Qiu, Bing-Long Shi, Shao-Juan Zhou, Kai-Chun Wu, Dai-Ming Fan, Institute of Digestive Diseases, Xijing Hospital, The Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China

Dao-Rong Peng, Department of Bacteriology, Xijing Hospital, The Fourth Military Medical University, Xi’an 710032, China

Author contributions: All authors contributed equally to the work.

Correspondence to: Jia-Lu Hu, Institute of Digestive Diseases, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China. jiwsh@netease.com

Telephone: +86-029-3375229 (lab), Fax: +86-29-7432505 (home)

Received: May 31, 2001
Revised: July 19, 2001
Accepted: August 1, 2001
Published online: December 15, 2001

Abstract

AIM: To study the polymorphism of flagellin A genotype and its significance in Helicobacter pylori (H. pylori).

METHODS: As the template, genome DNA was purified from six clinical isolates of H. pylori from outpatients, and the corresponding flagellin A fragments were amplified by polymerase chain reaction. All these products were sequenced. These sequences were compared with each other, and analyzed by software of FASTA program.

RESULTS: Specific PCR products were amplified from all of these H. pylori isolates and no length divergence was found among them. Compared with each other, the highest ungapped identity is 99.10%, while the lowest is 94.65%. Using FASTA program, the alignments between query and library sequences derived from different H. pylori strains were higher than 90%.

CONCLUSION: The nucleotide sequence of flagellin A in H. pylori is highly conservative with incident divergence. This information may be useful for gene diagnosis and further study on flagellar antigen phenotype.

Keywords: Helicobacter pylori; flagellin A; polymorphism; polymerase chain reaction