Original Articles
Copyright ©The Author(s) 1999. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Aug 15, 1999; 5(4): 324-326
Published online Aug 15, 1999. doi: 10.3748/wjg.v5.i4.324
Codon 249 mutations of p53 gene in non-neoplastic liver tissues
Xiao-Mou Peng, Chun-Lan Yao, Xue-Juan Chen, Wen-Wei Peng, Zhi-Liang Gao
Xiao-Mou Peng, Chun-Lan Yao, Xue-Juan Chen, Wen-Wei Peng, Zhi-Liang Gao, Department of Infectious Diseases, Third Affiliated Hospital, Sun Yat-Sen University of Medical Sciences, Guangzhou 510630, Guangdong Province, China
Author contributions: All authors contributed equally to the work.
Supported by the China Medical Board (CMB) of New York, Inc, Grant NO. 93-582
Correspondence to: Dr. Xiao-Mou Peng, Department of Infectious Diseases, Third Affiliated Hospital, Sun Yat-Sen University of Medical Sciences, Guangzhou 510630, Guangdong Province, China
Telephone: +86-20-85516867
Received: March 10, 1999
Revised: July 3, 1999
Accepted: July 19, 1999
Published online: August 15, 1999
Abstract

AIM: To study the significance of p53 gene in hepatocarcino genesis through analyzing codon 249 mutations of p53 gene in non-neoplastic liver tissues.

METHODS: Codon 249 mutation was detected using single-strande d conformational polymorphism analysis and allele-specific PCR in liver tissues from 10 cases of chronic hepatitis, 5 cases of cirrhosis and 20 cases of HCCs.

RESULTS: The detection rate of codon 249 mutation in chronic hepatitis, cirrhosis and pericancerous tissues was 70% (7/10), 100% (5/5) and 70% (14/20), respectively by AS-PCR. These mutations could not be detected b y SSCP analysis. The detection rates were 65% (13/20) and 45% (9/20) in cancerous tissues by AS-PCR and SSCP analysis.

CONCLUSION: Codon 249 mutations of p53 gene were very popular in non-neoplastic liver tissues though the number of those mutant cells was only in subsection. Those mutations in cancerous tissues might take place in the stage before the formation of tumor.

Keywords: liver; p53 gene; codon 249 mutation; liver neoplasms; hepatitis, viral; liver cirrhosis; polymerase chain reaction