Original Articles
Copyright ©The Author(s) 1998. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Feb 15, 1998; 4(1): 38-40
Published online Feb 15, 1998. doi: 10.3748/wjg.v4.i1.38
Expression of glutathione S-transferase placental mRNA in hepatic preneoplastic lesions in rats
Huan-Zhang Zhu, Xing-Li Zhang, Yi-Sheng Chen
Huan-Zhang Zhu, Xing-Li Zhang, Yi-Sheng Chen, Department of Pathology, Third Military Medical University, Chongqing 400038, China
Huan-Zhang Zhu, male, born on February 26, 1964 and graduated from Department of Medicine, Third Military Medical University, Lecturer of Pathology, having 4 papers published.
Author contributions: All authors contributed equally to the work.
Correspondence to: Dr. Huan-Zhang Zhu, Department of Pathology, Third Military Medical University, Chongqing 400038, China
Telephone: +86-23-68752281
Received: March 26, 1997
Revised: May 28, 1997
Accepted: July 26, 1997
Published online: February 15, 1998
Abstract

AIM: To detect glutathione S-transferase placental (GST-P) mRNA expression in hepatic preneoplastic lesions in rats.

METHODS: Using Solit-Farber model, the GST-P mRNA expression was observed in hepatic preneoplastic lesions induced by diethylnitrosamine (DEN) in rats and normal and regenerated hepatic tissues in the control group by in situ hybridization.

RESULTS: GST-P mRNA was mainly expressed in altered hepatic foci (AHF) and some of the oval cells in hepatic preneoplastic lesions and the extent of its expression was different among various foci or/and positive cells in the same focus whereas no expression was observed in normal and regenerated hepatic tissues.

CONCLUSION: Cells in AHF and oval cells may be the preneoplastic cells in the experimental hepatocellular carcinoma at the molecular level and heterogeneity exists in GST-P transcription levels.

Keywords: liver neoplasms; glutathione S-transferase; in situ hybridization; RNA, messenger; precancerous conditions