Basic Study
Copyright ©The Author(s) 2025. Published by Baishideng Publishing Group Inc. All rights reserved.
World J Gastroenterol. Feb 21, 2025; 31(7): 97599
Published online Feb 21, 2025. doi: 10.3748/wjg.v31.i7.97599
Protective effects of autologous bone marrow-derived mesenchymal stem cell transplantation on acute radioactive enteritis in Beagle dogs
Guang-Chen Sun, Wen-Da Xu, Hui Yao, Jiang Chen, Ruo-Nan Chai
Guang-Chen Sun, College of Medicine and Biological Information Engineering, Northeastern University, Shenyang 110000, Liaoning Province, China
Guang-Chen Sun, Ruo-Nan Chai, Department of Respiratory Medicine, General Hospital of Northern Theater Command, Shenyang 110000, Liaoning Province, China
Wen-Da Xu, Hui Yao, Jiang Chen, Department of Gastroenterology, General Hospital of Northern Theater Command, Shenyang 110000, Liaoning Province, China
Co-corresponding authors: Jiang Chen and Ruo-Nan Chai.
Author contributions: Sun GC, Chen J, and Xu WD conceptualized and designed the research; Sun GC and Yao H isolated the sample cells and performed cell culture and identification; Chen J and Xu WD established a disease model and evaluated relevant monitoring indicators; Chen J and Chai RN performed data analysis; Sun GC, Chen J, and Chai RN wrote the paper; all the authors have read and approved the final manuscript. Chen J applied for and obtained the funds for this research project. He conceptualized, designed, and supervised the whole process of the project. Chai RN is instrumental and responsible for data re-analysis and re-interpretation, figure plotting, comprehensive literature search, and preparation and revision of the manuscript. Both authors have made crucial and indispensable contributions towards the completion of the project and thus qualified as the co-corresponding authors of the paper.
Supported by People's Livelihood Science and Technology Plan Joint Project of Liaoning Province, No. 2021JH2/10300083.
Institutional animal care and use committee statement: The animal experiments were approved by the Animal Medical Research Ethics Committee of General Hospital of Northern Theater Command of PLA (Protocol No. 2023-15; April 14, 2023).
Conflict-of-interest statement: All the authors report no relevant conflicts of interest for this article.
ARRIVE guidelines statement: The authors have read the ARRIVE guidelines, and the manuscript was prepared and revised according to the ARRIVE guidelines.
Data sharing statement: The datasets involved in the current study are available from the corresponding author at matrix44@126.com on reasonable request.
Open Access: This article is an open-access article that was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution NonCommercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: https://creativecommons.org/Licenses/by-nc/4.0/
Corresponding author: Jiang Chen, PhD, Chief Physician, Professor, Department of Gastroenterology, General Hospital of Northern Theater Command, No. 83 Wenhua Road, Shenyang 110000, Liaoning Province, China. matrix44@126.com
Received: June 4, 2024
Revised: November 25, 2024
Accepted: December 18, 2024
Published online: February 21, 2025
Processing time: 230 Days and 3.9 Hours
Abstract
BACKGROUND

Radiation enteritis is a common complication of radiation therapy in which the surrounding normal intestinal tissue is damaged by ionising radiation, and there is no standard pharmacological prophylaxis or treatment regimen available. Mesenchymal stem cell transplantation can be used for radiation protection and the treatment of acute radiation injury, but its therapeutic mechanism of action remains unclear.

AIM

To investigate the protective effects of autologous bone marrow-derived mesenchymal stem cell (ABMSC) transplantation on radiation-induced intestinal injury.

METHODS

A model of acute radioactive enteritis was established in dogs by applying abdominal intensity-modulated radiation at a single X-ray dose of 12 Gy. ABMSCs were transplanted into the mesenteric artery with the technology of femoral artery puncture and DSA imaging two days after radiation. Visual and histopathological changes of the experimental dogs were observed. Different kinds of cytokines from intestinal samples were tested using Quantibody Canine Cytokine Array method. Enzyme-linked immunosorbent assay (ELISA) was also used to evaluate the cytokines changes in serum.

RESULTS

The ABMSCs group showed significant improvements in survival status compared with the blank and saline treatment groups. Histological observations revealed that the former had lower histological scores than the later after treatment (P < 0.05). Compared to the control groups, interleukin (IL)-10 and monocyte chemotactic protein (MCP)-1 from intestinal samples showed a remarkable increase and ELISA of serum samples proved higher secretion of the two target cytokines in the ABMSCs group (P < 0.05).

CONCLUSION

Our data suggest that transplantation of ABMSCs promotes intestinal recovery after acute radioactive injury in Beagle dogs. The cytokines of IL-10 and MCP-1 might play an important role in this process.

Keywords: Mesenchymal stem cells; Transplantation; Acute radioactive enteritis; Antibody array; Interleukin 10; Monocyte chemotactic protein-1

Core Tip: Mesenchymal stem cell transplantation has a therapeutic role in intestinal radiation injury, but the exact mechanism is unknown. In the present study, we explored the therapeutic role of autologous bone marrow-derived mesenchymal stem cells (ABMSCs) and their possible mechanisms in a Beagle model of radioactive intestinal injury. Our experimental results suggest that ABMSC transplantation has a protective effect against acute radiation enteritis in Beagle dogs and that interleukin-10 and monocyte chemotactic protein-1 may play an important role in the treatment of ABMSCs.