Published online Mar 28, 2017. doi: 10.3748/wjg.v23.i12.2149
Peer-review started: November 28, 2016
First decision: December 28, 2016
Revised: January 16, 2017
Accepted: February 16, 2017
Article in press: February 17, 2017
Published online: March 28, 2017
Processing time: 122 Days and 19.7 Hours
To determine whether oral administration of Bifidobacterium infantis CGMCC313-2 (B. infantis CGMCC313-2) inhibits allergen-induced airway inflammation and food allergies in a mouse model.
Ovalbumin (OVA)-induced allergic asthma and β-lactoglobulin-induced food allergy mouse models were used in this study. Following oral administration of B. infantis CGMCC313-2 during or after allergen sensitization, histopathologic changes in the lung and intestine were evaluated by hematoxylin and eosin (HE) staining. In the allergic asthma mouse model, we evaluated the proportion of lung-infiltrating inflammatory cells. OVA-specific IgE and IgG1 levels in serum and cytokine levels in bronchoalveolar lavage fluid (BALF) were also assessed. In the food allergy mouse model, the levels of total IgE and cytokines in serum were measured.
Oral administration of B. infantis CGMCC313-2 during or after allergen sensitization suppressed allergic inflammation in lung and intestinal tissues, while the proportion of infiltrating inflammatory cells was significantly decreased in the BALF of allergic asthma mice. Moreover, B. infantis CGMCC313-2 decreased the serum levels of total IgE in food allergy mice, and reductions in IgE and IgG1 were also observed in OVA-induced allergic asthma mice. The expression of interleukin-4 (IL-4) and IL-13 in both serum and BALF was suppressed following the administration of B. infantis CGMCC313-2, while an effect on serum IL-10 levels was not observed.
B. infantis CGMCC313-2 inhibits the secretion of allergen-induced IgE, IL-4 and IL-13, and attenuates allergic inflammation.
Core tip:Bifidobacterium infantis CGMCC313-2 significantly decreased the serum concentration of IgE and IgG1 in asthma and food allergy mouse models. The number of infiltrating cells in bronchoalveolar lavage fluid was reduced, and eosinophil infiltration in lungs was relieved by B. infantis CGMCC313-2 in allergic asthma mice. Body weight was regained in food allergy mice, and intestinal inflammation was attenuated by B. infantis CGMCC313-2. Following administration of B. infantis CGMCC313-2, the concentrations of interleukin-4 (IL-4) and IL-13 decreased in both allergic asthma and food allergy mice.