Expression profile of microRNAs in gastrointestinal stromal tumors revealed by high throughput quantitative RT-PCR microarray

doi:10.3748/wjg.v21.i19.5843

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World Journal of Gastroenterology

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World J Gastroenterol. May 21, 2015; 21(19): 5843-5855
Published online May 21, 2015. doi: 10.3748/wjg.v21.i19.5843

Expression profile of microRNAs in gastrointestinal stromal tumors revealed by high throughput quantitative RT-PCR microarray

Han-Xing Tong, Yu-Hong Zhou, Ying-Yong Hou, Yong Zhang, Yuan Huang, Bin Xie, Jiong-Yuan Wang, Quan Jiang, Jun-Yi He, Ye-Bo Shao, Wu-Mei Han, Ruo-Ying Tan, Jun Zhu, Wei-Qi Lu

Han-Xing Tong, Yong Zhang, Jiong-Yuan Wang, Quan Jiang, Jun-Yi He, Ye-Bo Shao, Jun Zhu, Wei-Qi Lu, Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai 200032, China

Yu-Hong Zhou, Department of Oncology, Zhongshan Hospital, Fudan University, Shanghai 200032, China

Ying-Yong Hou, Department of Pathology, Zhongshan Hospital, Fudan University, Shanghai 200032, China

Yuan Huang, The Center of Endoscopy, Zhongshan Hospital, Fudan University, Shanghai 200032, China

Bin Xie, Department of Surgery, The Central Hospital of Zaozhuang Mining Group, Zaozhuang 277000, Shandong Province, China

Wu-Mei Han, Ruo-Ying Tan, Biovue Technology Ltd, Shanghai 200032, China

Author contributions: Tong HX, Zhou YH, Hou YY and Zhang Y contributed equally to this work; Tong HX, Zhou YH, Hou YY, Lu WQ and Zhang Y designed the research plan; Tong HX, Zhou YH, Hou YY, Han WM, Xie B, Wang JY, Jiang Q, He JY and Shao YB performed the research; Tong HX, Tan RY, Zhang Y, Zhu J and Lu WQ analyzed the data; and Huang Y and Tong HX wrote the manuscript.

Supported by Grants from the Ministry of Health of the China, No. W2012RQ02; Shanghai Science and Technology Committee, No. 12nm0501402; and Shanghai Education Committee, No. 120311.

Correspondence to: Wei-Qi Lu, MD, Department of General Surgery, Zhongshan Hospital, Fudan University, 180 Fenglin Road, Shanghai, 200032, China. lu.weiqi@zs-hospital.sh.cn

Telephone: +86-21-64041990 Fax: +86-21-64041990

Received: July 7, 2014
Peer-review started: July 8, 2014
First decision: August 12, 2014
Revised: September 1, 2014
Accepted: December 1, 2014
Article in press: December 1, 2014
Published online: May 21, 2015
Processing time: 317 Days and 5.3 Hours

Abstract

AIM: To investigate the microRNA (miRNA) expression profile in gastrointestinal stromal tumor (GIST) tissues that could serve as a novel diagnostic biomarker for GIST detection.

METHODS: We performed a quantitative real-time quantitative reverse transcriptase polymerase chain reaction assay to analyze the expression of 1888 miRNAs in a sample set that included 54 GIST tissue samples.

RESULTS: We found that dysregulation of several miRNAs may be related to the malignant potential of GISTs. Six of these miRNAs, hsa-let-7c, miR-218, miR-488#, miR-4683, miR-34c-5p and miR-4773, were selected as the final list of biomarkers to separate the malignant GISTs (M group) from the benign GISTs (B group). In addition, MiR-29b-2#, hsa-let-7c, miR-891b, miR-218, miR-204, miR-204-3p, miR-628-5p, miR-744, miR-29c#, miR-625 and miR-196a were used to distinguish between the borderline (BO group) and M groups. There were 11 common miRNAs selected to separate the benign and borderline (BB) group from the M group, including hsa-let-7c, miR-218, miR-628-5p, miR-204-3p, miR-204, miR-891b, miR-488#, miR-145, miR-891a, miR-34c-5p and miR-196a.

CONCLUSION: The identified miRNAs appear to be novel biomarkers to distinguish malignant from benign GISTs, which may be helpful to understand the mechanisms of GIST oncogenesis and progression, and to further elucidate the characteristics of GIST subtypes.

Keywords: Gastrointestinal stromal tumors; MicroRNAs; Microarray analysis; Real-time polymerase chain reaction; Diagnosis

Core tip: Using high throughput quantitative reverse transcription-polymerase chain reaction microarray, we obtained a panel of miRNAs including hsa-let-7c, miR-218, miR-488#, miR-4683, miR-34c-5p and miR-4773, which can distinguish malignant from benign gastrointestinal stromal tumors (GISTs). Understanding the mechanisms of GIST tumorigenesis and development, may help to further elucidate the characteristics of GIST subtypes.