Published online Apr 14, 2015. doi: 10.3748/wjg.v21.i14.4178
Peer-review started: October 31, 2014
First decision: November 14, 2014
Revised: December 9, 2014
Accepted: January 16, 2015
Article in press: January 16, 2015
Published online: April 14, 2015
Processing time: 166 Days and 19.3 Hours
AIM: To investigate the bactericidal effects of Chenopodium ambrosioides L. (CAL) against Helicobacter pylori (H. pylori) both in vitro and in vivo.
METHODS: For in vitro experiments, the inhibitory activity of CAL was tested using an agar dilution method; H. pylori strain NCTC11637 was incubated on Columbia blood agar plates containing serial concentrations of CAL. The minimal inhibitory concentration (MIC) was determined by the absence of H. pylori colonies on the agar plate. Time-kill curves were used to evaluate bactericidal activity; the average number of colonies was calculated at 0, 2, 8 and 24 h after liquid incubation with concentrations of CAL at 0.5, 1, and 2 × MIC. For in vivo experiments, H. pylori-infected mice were randomly divided into CAL, triple therapy (lansoprazole, metronidazole, and clarithromycin), blank control, or H. pylori control groups. The eradication ratios were determined by positive findings from rapid urease tests (RUTs) and by histopathology.
RESULTS: In vitro, the MIC of CAL against H. pylori was 16 mg/L. The time-kill curves showed a stable and persistent decreasing tendency with increasing CAL concentration, and the intensity of the bactericidal effect was proportional to dose; the 1 and 2 × MIC completely inhibited the growth of H. pylori at 24 h. In vivo, the eradication ratios in the CAL group were 60% (6/10) by RUT and 50% (5/10) by histopathology. Ratios in the triple therapy group were both 70% (7/10), and there was no difference between the CAL and triple therapy groups. Histopathologic evaluation revealed massive bacterial colonization on the surface of gastric mucosa and slight infiltration of mononuclear cells after inoculation with H. pylori, but no obvious inflammation or other pathologic changes in gastric mucosa of mice from CAL and triple therapy groups.
CONCLUSION: CAL demonstrates effective bactericidal activity against H. pylori both in vitro and in vivo.
Core tip: The Helicobacter pylori (H. pylori) eradication rate of triple therapy has been markedly decreased due to increasing bacterial antibiotic resistance. Natural Chinese medicines, such as Chenopodium ambrosioides L. (a Chinese herb derived from Jinhua Weikang Capsule utilized for gastritis and peptic ulcers), represent complementary and collaborative therapies. This report demonstrates that C. ambrosioides has in vitro and in vivo bactericidal effects against H. pylori, and may be a good candidate for the treatment of H. pylori infection.