Research Report
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World J Gastroenterol. Dec 7, 2014; 20(45): 17092-17099
Published online Dec 7, 2014. doi: 10.3748/wjg.v20.i45.17092
Inhibitory effects of dobutamine on human gastric adenocarcinoma
Hui-Xia Zheng, Li-Na Wu, Hong Xiao, Qian Du, Jian-Fang Liang
Hui-Xia Zheng, Li-Na Wu, Hong Xiao, Qian Du, Jian-Fang Liang, Department of Pathology, The First Hospital, Shanxi Medical University, Taiyuan 030024, Shanxi Province, China
Author contributions: Zheng HX and Wu LN contributed equally to this work; Zheng HX, Wu LN and Liang JF designed the research; Wu LN, Xiao H and Du Q performed the research; Xiao H and Du Q analyzed the data; Wu LN, Du Q and Liang JF wrote the paper.
Correspondence to: Jian-Fang Liang, MD, Chief Physician, Department of Pathology, The First Hospital, Shanxi Medical University, No. 85 South Jiefang Road, Taiyuan 030024, Shanxi Province, China. liangjianfang2000@163.com
Telephone: +86-351-4639499 Fax: +86-351-8263028
Received: February 24, 2014
Revised: May 29, 2014
Accepted: July 11, 2014
Published online: December 7, 2014
Processing time: 289 Days and 3.6 Hours
Abstract

AIM: To explore the inhibitory effects of dobutamine on gastric adenocarcinoma cells.

METHODS: Dobutamine was used to treat gastric adenocarcinoma cells (SGC-7901) and cell viability was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The effects of dobutamine combined with cisplatin on cell viability were also analyzed. Cell migration was studied using the wound healing assay, and cell proliferation was analyzed using the colony formation assay. A cell invasion assay was carried out using Transwell cell culture chambers. The cell cycle and cell apoptosis were analyzed by flow cytometry. Western blot and immunocytochemistry were performed to determine the expression of Yes-associated protein (YAP) in treated cells.

RESULTS: Dobutamine significantly inhibited cell growth, migration, cell colony formation, and cell invasion into Matrigel. Dobutamine also arrested the cell cycle at G1/S phase, and increased the rate of apoptosis of gastric adenocarcinoma cells. The expression of YAP was detected mainly in the nucleus in the absence of dobutamine. However, reduced expression of phosphorylated YAP was mainly found in the cytosol following treatment with dobutamine.

CONCLUSION: Dobutamine has significant inhibitory effects on gastric adenocarcinoma cells and may be used in neoadjuvant therapy not only for gastric cancer, but also for other tumors.

Keywords: Dobutamine; Gastric adenocarcinoma cells; Yes-associated protein; Hippo pathway; Human gastric adenocarcinoma cell line SGC-7901; Therapy

Core tip: Dobutamine inhibited the viability, migration, proliferation and invasion of SGC-7901 cells. Dobutamine arrested the cell cycle at G1/S phase, and increased the rate of apoptosis of gastric adenocarcinoma cells. Phosphorylated Yes-associated protein was found mainly in the cytosol after treatment with dobutamine.