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Publication Name
World Journal of Gastroenterology
ISSN
1007-9327
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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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©2013 Baishideng Publishing Group Co., Limited. All rights reserved.
World J Gastroenterol. Mar 28, 2013; 19(12): 1919-1929
Published online Mar 28, 2013. doi: 10.3748/wjg.v19.i12.1919
Immunohistochemical study of the digestive tract of Oligosarcus hepsetus
Danielle A Vieira-Lopes, Nadja L Pinheiro, Armando Sales, Adriana Ventura, Francisco G Araújo, Iracema D Gomes, Aparecida A Nascimento
Danielle A Vieira-Lopes, Nadja L Pinheiro, Armando Sales, Adriana Ventura, Francisco G Araújo, Iracema D Gomes, Aparecida A Nascimento, Departamento de Biologia Animal, Instituto de Biologia, Universidade Federal Rural do Rio de Janeiro, Seropédica, Rio de Janeiro 23890-000, Brazil
Author contributions: Vieira-Lopes DA, Pinheiro NL, Sales A, Ventura A, Araújo FG, Gomes ID and Nascimento AA designed the research; Vieira-Lopes DA, Ventura A, Gomes ID and Nascimento AA performed the research; Vieira-Lopes DA, Pinheiro NL, Sales A, Araújo FG and Nascimento AA analyzed the data; Vieira-Lopes DA and Nascimento AA wrote the paper.
Supported by Federal Rural University of Rio de Janeiro for the Research Grant under the Internal Scientific Initiation Program, to Vieira-Lopes DA
Correspondence to: Dr. Danielle Vieira-Lopes, Departamento de Biologia Animal, Instituto de Biologia, Universidade Federal Rural do Rio de Janeiro, Seropédica, Rio de Janeiro 23890-000, Brazil. dmjvieira@hotmail.com
Telephone: +55-21-26821711 Fax: +55-21-26821711
Received: November 8, 2012
Revised: November 26, 2012
Accepted: December 25, 2012
Published online: March 28, 2013
Processing time: 163 Days and 9.2 Hours
Abstract

AIM: To describe the histology of the digestive tract and to investigate the occurrence of endocrine cells in Oligosarcus hepsetus (O. hepsetus).

METHODS: The digestive tract (DT) of O. hepsetus was divided into esophagus, two stomach regions (glandular and non-glandular) and two intestinal regions (anterior and posterior). These specimens were processed by routine histological techniques and stained with hematoxylin-eosin, Gomori’s trichrome, periodic acid Schiff (PAS) and Alcian blue (AB). An immunohistochemical method using avidin-biotin-peroxidase was employed.

RESULTS: The esophagus is lined with a non-keratinized stratified squamous epithelium that is reactive to PAS and AB. The stomach has a mucosa lined with a simple columnar epithelium with mucus-secreting cells that are reactive only to PAS. The intestine has a simple columnar epithelium with a brush border and goblet cells that are reactive to PAS and AB. Somatostatin, serotonin and cholecystokinin immunoreactive cells were identified throughout the DT.

CONCLUSION: This study revealed adaptations for the species’ diet and showed that the distribution and relative frequency of immunoreactive cells are similar to those of other fish.

Keywords: Fish; Esophagus; Stomach; Intestine; Endocrine cells
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