Brief Article
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World J Gastroenterol. Dec 28, 2012; 18(48): 7285-7289
Published online Dec 28, 2012. doi: 10.3748/wjg.v18.i48.7285
Growth-inhibitory effects of MOB2 on human hepatic carcinoma cell line SMMC-7721
Jian-Jun Leng, Hua-Min Tan, Ke Chen, Wei-Gan Shen, Jing-Wang Tan
Jian-Jun Leng, Jing-Wang Tan, Institute of Hepatobiliary Surgery, PLA General Hospital, Beijing 100853, China
Hua-Min Tan, Department of General Surgery, Fuzhou General Hospital of Nanjing Military Command, Fuzhou, Jiangsu Province, China
Ke Chen, Department of Hepato-bilio-pancreatic Surgery, Northern Jiangsu People‘s Hospital, Yangzhou 225001, Jiangsu Province, China
Wei-Gan Shen, Department of Molecular Biology Laboraoty, Medical College of Yangzhou University, Yangzhou 225001, Jiangsu Province, China
Author contributions: This study was designed by Tan JW; Leng JJ, Tan HM and Chen K carried out the majority of the experiments and performed the data analysis; Shen WG supervised experimental work; the manuscript was written by Tan JW who is full responsibility for the conduct of the study; and all authors have read and approved the final manuscript.
Correspondence to: Jing-Wang Tan, Professor, Institute of Hepatobiliary Surgery, PLA General Hospital, 28 Fuxing Road, Haidian District, Beijing 100853, China. tanjingwang02@yahoo.com.cn
Telephone: +86-10-85272608 Fax: +86-10-87370004
Received: June 27, 2011
Revised: August 8, 2011
Accepted: August 15, 2011
Published online: December 28, 2012
Abstract

AIM: To investigate the growth-inhibiting and apoptosis-inducing effects of the gene MOB2 on human hepatic carcinoma cell line SMMC-7721.

METHODS: The full-length cDNA of the MOB2 gene was amplified from human umbilical vein endothelial cells. The correct full-length MOB2 cDNA was subcloned into the eukaryotic expression vector pEGFP-C1. After lipofection of the MOB2 gene into cancer cells, the levels of MOB2 protein in the cancer cells were detected by immunoblotting. To transfect the recombined plasmid vector pEGFP-CI-MOB2 into SMMC-7721 cells, the cells were cultured in Dulbecco’s Modified Eagle’s Medium with 10% fetal calf serum and glutamine, and then mixed with liposomes, Lipofectamine 2000 and the plasmid vector pEGFP-CI-MOB2.

RESULTS: We observed the growth and proliferation of SMMC-7721 cells containing pEGFP-CI-MOB2 and analyzed their apoptosis and growth cycle phases by flow cytometry. We successfully transfected the recombined plasmid vector pEGFP-CI-MOB2 into SMMC-7721 cells and screened for a single clone cell containing MOB2. After transfection, MOB2 enhanced growth suppression, induced apoptosis, increased the ratio of G0/G1, significantly inhibited the advance of cell cycle phase, and arrested cells in G0/G1 phase.

CONCLUSION: MOB2 overexpression induces apoptosis and inhibits the growth of human hepatic cancer cells, which may be useful in gene therapy for hepatic carcinoma.

Keywords: Gene expression; SMMC-7721; Growth inhibition; Apoptosis