Chen YH, Lan T, Li J, Qiu CH, Wu T, Gou HJ, Lu MQ. Gardenia jasminoides attenuates hepatocellular injury and fibrosis in bile duct-ligated rats and human hepatic stellate cells. World J Gastroenterol 2012; 18(48): 7158-7165 [PMID: 23326120 DOI: 10.3748/wjg.v18.i48.7158]
Corresponding Author of This Article
Min-Qiang Lu, MD, Professor, Department of Hepatology, The Third Affiliated Hospital of Sun Yat-sen University, No. 600 Tianhe Road, Guangzhou 510630, Guangdong Province, China. lantiansci@yahoo.com
Article-Type of This Article
Original Article
Open-Access Policy of This Article
This article is an open-access article which was selected by an in-house editor and fully peer-reviewed by external reviewers. It is distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/
Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA
Share the Article
Chen YH, Lan T, Li J, Qiu CH, Wu T, Gou HJ, Lu MQ. Gardenia jasminoides attenuates hepatocellular injury and fibrosis in bile duct-ligated rats and human hepatic stellate cells. World J Gastroenterol 2012; 18(48): 7158-7165 [PMID: 23326120 DOI: 10.3748/wjg.v18.i48.7158]
World J Gastroenterol. Dec 28, 2012; 18(48): 7158-7165 Published online Dec 28, 2012. doi: 10.3748/wjg.v18.i48.7158
Gardenia jasminoides attenuates hepatocellular injury and fibrosis in bile duct-ligated rats and human hepatic stellate cells
Ying-Hua Chen, Tian Lan, Jing Li, Chun-Hui Qiu, Teng Wu, Hong-Ju Gou, Min-Qiang Lu
Ying-Hua Chen, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China
Tian Lan, Teng Wu, Hong-Ju Gou, Institute of Vascular Biology, Guangdong Pharmaceutical University, Guangzhou 510006, Guangdong Province, China
Jing Li, Guangzhou PTDS Biotechnology Science and Technology Co. Ltd., Guangzhou 510540, Guangdong Province, China
Chun-Hui Qiu, Min-Qiang Lu, Department of Hepatology, The Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, Guangdong Province, China
Author contributions: Chen YH and Lan T designed and performed the study, collected and analyzed the data, wrote and revised the manuscript, and contributed equally to this work; Li J and Qiu CH contributed to the acquisition of data; Wu T and Gou HJ participated in the study design and experiments; Lu MQ contributed to the conception and design of the study as well as funding application; and all authors have approved the final version to be published.
Supported by The Natural Science Foundation of China, No. 81170450 to Lu MQ and No. 81200308 to Lan T; The PhD Start-up Fund of Natural Science Foundation of Guangdong Province, China, No. S2012040008026; and The New Star of Science and Technology Foundation of Zhu Jiang in Guangzhou City
Correspondence to: Min-Qiang Lu, MD, Professor, Department of Hepatology, The Third Affiliated Hospital of Sun Yat-sen University, No. 600 Tianhe Road, Guangzhou 510630, Guangdong Province, China. lantiansci@yahoo.com
Telephone: +86-20-87333774 Fax: +86-20-87331220
Received: June 19, 2012 Revised: October 10, 2012 Accepted: October 16, 2012 Published online: December 28, 2012
Abstract
AIM: To investigate the anti-hepatofibrotic effects of Gardenia jasminoides in liver fibrosis.
METHODS: Male Sprague-Dawley rats underwent common bile duct ligation (BDL) for 14 d and were treated with Gardenia jasminoides by gavage. The effects of Gardenia jasminoides on liver fibrosis and the detailed molecular mechanisms were also assessed in human hepatic stellate cells (LX-2) in vitro.
RESULTS: Treatment with Gardenia jasminoides decreased serum alanine aminotransferase (BDL vs BDL + 100 mg/kg Gardenia jasminoides, 146.6 ± 15 U/L vs 77 ± 6.5 U/L, P = 0.0007) and aspartate aminotransferase (BDL vs BDL + 100 mg/kg Gardenia jasminoides, 188 ± 35.2 U/L vs 128 ± 19 U/L, P = 0.005) as well as hydroxyproline (BDL vs BDL + 100 mg/kg Gardenia jasminoides, 438 ± 40.2 μg/g vs 228 ± 10.3 μg/g liver tissue, P = 0.004) after BDL. Furthermore, Gardenia jasminoides significantly reduced liver mRNA and/or protein expression of transforming growth factor β1 (TGF-β1), collagen type I (Col I) and α-smooth muscle actin (α-SMA). Gardenia jasminoides significantly suppressed the upregulation of TGF-β1, Col I and α-SMA in LX-2 exposed to recombinant TGF-β1. Moreover, Gardenia jasminoides inhibited TGF-β1-induced Smad2 phosphorylation in LX-2 cells.
CONCLUSION: Gardenia jasminoides exerts antifibrotic effects in the liver fibrosis and may represent a novel antifibrotic agent.
