Original Article
Copyright ©2012 Baishideng Publishing Group Co., Limited. All rights reserved.
World J Gastroenterol. Nov 7, 2012; 18(41): 5889-5896
Published online Nov 7, 2012. doi: 10.3748/wjg.v18.i41.5889
Characteristics of deslanoside-induced modulation on jejunal contractility
Da-Peng Chen, Yong-Jian Xiong, Ze-Yao Tang, Qi-Ying Yao, Dong-Mei Ye, Sha-Sha Liu, Yuan Lin
Da-Peng Chen, Yong-Jian Xiong, Ze-Yao Tang, Qi-Ying Yao, Dong-Mei Ye, Sha-Sha Liu, Yuan Lin, Department of Pharmacology, Dalian Medical University, Dalian 116044, Liaoning Province, China
Author contributions: Chen DP designed and performed the research and wrote the paper; Tang ZY, Yao QY and Ye DM designed the research; Liu SS and Xiong YJ performed the research; Lin Y supervised the study and revised the paper.
Supported by National Natural Science Foundation of China, No. 30772601
Correspondence to: Yuan Lin, PhD, Department of Pharmacology, Dalian Medical University, Dalian 116044, Liaoning Province, China. linyuandmu2008@qq.com
Telephone: +86-411-86110409 Fax: +86-411-86110409
Received: April 22, 2012
Revised: July 3, 2012
Accepted: August 14, 2012
Published online: November 7, 2012
Abstract

AIM: To characterize the dual effects of deslanoside on the contractility of jejunal smooth muscle.

METHODS: Eight pairs of different low and high contractile states of isolated jejunal smooth muscle fragment (JSMF) were established. Contractile amplitude of JSMF in different low and high contractile states was selected to determine the effects of deslanoside, and Western blotting analysis was performed to measure the effects of deslanoside on myosin phosphorylation of jejunal smooth muscle.

RESULTS: Stimulatory effects on the contractility of JSMF were induced (45.3% ± 4.0% vs 87.0% ± 7.8%, P < 0.01) by deslanoside in 8 low contractile states, and inhibitory effects were induced (180.6% ± 17.8% vs 109.9% ± 10.8%, P < 0.01) on the contractility of JSMF in 8 high contractile states. The effect of deslanoside on the phosphorylation of myosin light chain of JSMF in low (78.1% ± 4.1% vs 96.0% ± 8.1%, P < 0.01) and high contractile state (139.2% ± 8.5% vs 105.5 ± 7.34, P < 0.01) was also bidirectional. Bidirectional regulation (BR) was abolished in the presence of tetrodotoxin. Deslanoside did not affect jejunal contractility pretreated with the Ca2+ channel blocker verapamil or in a Ca2+-free assay condition. The stimulatory effect of deslanoside on JSMF in a low contractile state (low Ca2+ induced) was abolished by atropine. The inhibitory effect of deslanoside on jejunal contractility in a high contractile state (high Ca2+ induced) was blocked by phentolamine, propranolol and L-NG-nitro-arginine, respectively.

CONCLUSION: Deslanoside-induced BR is Ca2+ dependent and is related to cholinergic and adrenergic systems when JSMF is in low or high contractile states.

Keywords: Deslanoside; Bidirectional regulation; Contractile state; Jejunal smooth muscle