Li LZ, Deng HX, Lou WZ, Sun XY, Song MW, Tao J, Xiao BX, Guo JM. Growth inhibitory effect of 4-phenyl butyric acid on human gastric cancer cells is associated with cell cycle arrest. World J Gastroenterol 2012; 18(1): 79-83 [PMID: 22228974 DOI: 10.3748/wjg.v18.i1.79]
Corresponding Author of This Article
Dr. Jun-Ming Guo, Department of Biochemistry and Molecular Biology, Ningbo University School of Medicine, 818 Fenghua Road, Ningbo 315211, Zhejiang Province, China. junmingguo@yahoo.com
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Li LZ, Deng HX, Lou WZ, Sun XY, Song MW, Tao J, Xiao BX, Guo JM. Growth inhibitory effect of 4-phenyl butyric acid on human gastric cancer cells is associated with cell cycle arrest. World J Gastroenterol 2012; 18(1): 79-83 [PMID: 22228974 DOI: 10.3748/wjg.v18.i1.79]
Long-Zhu Li, Hong-Xia Deng, Wen-Zhu Lou, Xue-Yan Sun, Meng-Wan Song, Jing Tao, Bing-Xiu Xiao, Jun-Ming Guo, Department of Biochemistry and Molecular Biology, Ningbo University School of Medicine, Ningbo 315211, Zhejiang Province, China
Author contributions: Li LZ performed the research and drafted the paper; Deng HX, Lou WZ, Sun XY, Song MW and Tao J assisted in the research and revision of the paper; Xiao BX provided advice regarding the performance of the research; Guo JM designed the research and revised and contributed to the writing of the paper.
Supported by Natural Science Foundation of Ningbo, No. 2009A610134; Natural Sciences Foundation of Zhejiang, No. Y207244; College Students’ Science-Technology Innovation Program of Zhejiang Province, No. 200959; the Excellent Dissertation Foundation of Ningbo University, No. 201014; KC Wong Magna Fund of Ningbo University; the Scientific Innovation Team Project of Ningbo, No.2011B82014
Correspondence to: Dr. Jun-Ming Guo, Department of Biochemistry and Molecular Biology, Ningbo University School of Medicine, 818 Fenghua Road, Ningbo 315211, Zhejiang Province, China. junmingguo@yahoo.com
Telephone: +86-574-87600758 Fax: +86-574-87608638
Received: April 17, 2011 Revised: June 21, 2011 Accepted: June 28, 2011 Published online: January 7, 2012
Abstract
AIM: To investigate the growth effects of 4-phenyl butyric acid (PBA) on human gastric carcinoma cells and their mechanisms.
METHODS: Moderately-differentiated human gastric carcinoma SGC-7901 and lowly-differentiated MGC-803 cells were treated with 5, 10, 20, 40, and 60 μmol/L PBA for 1-4 d. Cell proliferation was detected using the MTT colorimetric assay. Cell cycle distributions were examined using flow cytometry.
RESULTS: The proliferation of gastric carcinoma cells was inhibited by PBA in a dose- and time-dependent fashion. Flow cytometry showed that SGC-7901 cells treated with low concentrations of PBA were arrested at the G0/G1 phase, whereas cells treated with high concentrations of PBA were arrested at the G2/M phase. Although MGC-803 cells treated with low concentrations of PBA were also arrested at the G0/G1 phase, cells treated with high concentrations of PBA were arrested at the S phase.
CONCLUSION: The growth inhibitory effect of PBA on gastric cancer cells is associated with alteration of the cell cycle. For moderately-differentiated gastric cancer cells, the cell cycle was arrested at the G0/G1 and G2/M phases. For lowly-differentiated gastric cancer cells, the cell cycle was arrested at the G0/G1 and S phases.
