Liu JB, Qiang FL, Dong J, Cai J, Zhou SH, Shi MX, Chen KP, Hu ZB. Plasma DNA methylation of Wnt antagonists predicts recurrence of esophageal squamous cell carcinoma. World J Gastroenterol 2011; 17(44): 4917-4921 [PMID: 22171134 DOI: 10.3748/wjg.v17.i44.4917]
Corresponding Author of This Article
Ke-Ping Chen, Professor, Institute of Life Science, Jiangsu University, Zhenjiang 212013, Jiangsu Province, China. kpchen@ujs.edu.cn
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Brief Article
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World J Gastroenterol. Nov 28, 2011; 17(44): 4917-4921 Published online Nov 28, 2011. doi: 10.3748/wjg.v17.i44.4917
Plasma DNA methylation of Wnt antagonists predicts recurrence of esophageal squamous cell carcinoma
Ji-Bin Liu, Fu-Lin Qiang, Jing Dong, Jin Cai, Shu-Hui Zhou, Min-Xin Shi, Ke-Ping Chen, Zhi-Bin Hu
Ji-Bin Liu, Ke-Ping Chen, Institute of Life Science, Jiangsu University, Zhenjiang 212013, Jiangsu Province, China
Ji-Bin Liu, Fu-Lin Qiang, Jin Cai, Min-Xin Shi, Nantong Tumor Hospital, Nantong 226361, Jiangsu Province, China
Jing Dong, Zhi-Bin Hu, Department of Epidemiology and Biostatistics, Ministry of Education Key Lab for Cancer, Nanjing Medical University, Nanjing 210029, Jiangsu Province, China
Shu-Hui Zhou, School of Radiology and Public Health, Soochow University, Suzhou 215123, Jiangsu Province, China
Zhi-Bin Hu, Jiangsu Key Lab of Cancer Biomarkers, Prevention and Treatment, Cancer Center, Nanjing 210029, Jiangsu Province, China
Author contributions: Liu JB and Qiang FL contributed equally to this work; Liu JB and Qiang FJ performed the majority of experiments; Liu JB, Qiang FJ, and Dong J provided vital reagents and analytical tools and edited the manuscript; Liu JB, Qiang FJ, Cai J, Zhou SH and Shi MX collected all the specimens for this work; Liu JB, Dong J, Chen KP, and Hu Z designed the study and wrote the manuscript.
Supported by Jiangsu University and Nantong Tumour Hospital
Correspondence to: Ke-Ping Chen, Professor, Institute of Life Science, Jiangsu University, Zhenjiang 212013, Jiangsu Province, China. kpchen@ujs.edu.cn
Telephone: +86-511-88791923 Fax: +86-511-88791923
Received: May 6, 2011 Revised: August 6, 2011 Accepted: August 13, 2011 Published online: November 28, 2011
Abstract
AIM: To detect the effects of plasma DNA methylation of Wnt antagonists/inhibitors on recurrence of esophageal squamous cell carcinoma (ESCC).
METHODS: We used methylation-specific polymerase chain reaction to detect hypermethylation of the promoter of four Wnt antagonists/inhibitors (SFRP-1, WIF-1, DKK-3 and RUNX3) using DNA from the plasma of ESCC patients (n = 81) and analyzed the association between promoter hypermethylation of Wnt pathway modulator genes and the two-year recurrence of ESCC.
RESULTS: Hypermethylation of SFRP-1, DKK-3 and RUNX-3 was significantly associated with an increased risk of ESCC recurrence (P = 0.001, 0.003 and 0.001 for SFRP-1, DKK-3 and RUNX3, respectively). Patients carrying two to three methylated genes had a significantly elevated risk of recurrence compared with those not carrying methylated genes (odds ratio = 15.69, 95% confidential interval: 2.97-83). The area under the receiver operating characteristic curve (AUC) was 77.1 for ESCC recurrence prediction (sensitivity = 66.67 and specificity = 83.3). When combining methylated genes and the clinical stage, the AUC was 83.69, with a sensitivity of 76.19 and a specificity of 83.3.
CONCLUSION: The status of promoter hypermethylation of Wnt antagonists/inhibitors in plasma may serve as a non-invasive prognostic biomarker for ESCC.
Liu JB, Qiang FL, Dong J, Cai J, Zhou SH, Shi MX, Chen KP, Hu ZB. Plasma DNA methylation of Wnt antagonists predicts recurrence of esophageal squamous cell carcinoma. World J Gastroenterol 2011; 17(44): 4917-4921 [PMID: 22171134 DOI: 10.3748/wjg.v17.i44.4917]
