Ruyssers NE, De Winter BY, De Man JG, Ruyssers ND, Van Gils AJ, Loukas A, Pearson MS, Weinstock JV, Pelckmans PA, Moreels TG. Schistosoma mansoni proteins attenuate gastrointestinal motility disturbances during experimental colitis in mice. World J Gastroenterol 2010; 16(6): 703-712 [PMID: 20135718 DOI: 10.3748/wjg.v16.i6.703]
Corresponding Author of This Article
Tom G Moreels, Professor, Division of Gastroenterology and Hepatology, Antwerp University Hospital, Wilrijkstraat 10, 2650 Antwerp, Belgium. tom.moreels@uza.be
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Original Article
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World J Gastroenterol. Feb 14, 2010; 16(6): 703-712 Published online Feb 14, 2010. doi: 10.3748/wjg.v16.i6.703
Schistosoma mansoni proteins attenuate gastrointestinal motility disturbances during experimental colitis in mice
Nathalie E Ruyssers, Benedicte Y De Winter, Joris G De Man, Natacha D Ruyssers, Ann J Van Gils, Alex Loukas, Mark S Pearson, Joel V Weinstock, Paul A Pelckmans, Tom G Moreels
Nathalie E Ruyssers, Benedicte Y De Winter, Joris G De Man, Natacha D Ruyssers, Ann J Van Gils, Paul A Pelckmans, Tom G Moreels, Laboratory of Experimental Medicine and Pediatrics, Division of Gastroenterology, University of Antwerp, 2610 Antwerp, Belgium
Alex Loukas, Mark S Pearson, Division of Infectious Diseases and Immunology, Queensland Institute of Medical Research, 4006 Brisbane, Australia
Joel V Weinstock, Division of Gastroenterology-Hepatology, Tufts New England Medical Center, Boston, MA 02111, United States
Author contributions: Ruyssers NE, De Winter BY, De Man JG, Ruyssers ND and Van Gils AJ performed the experiments; Ruyssers NE analyzed the data; Loukas A, Pearson MS and Weinstock JV provided the proteins and the analytic tools and were also involved in editing the manuscript; De Winter BY, De Man JG, Pelckmans PA and Moreels TG coordinated the work and were also involved in editing the manuscript; Ruyssers NE wrote the paper.
Supported by The Fund of Scientific Research (FWO), Flanders, Project G.0134.07 and by the University of Antwerp, BOF Grant FA02/3/3257
Correspondence to: Tom G Moreels, Professor, Division of Gastroenterology and Hepatology, Antwerp University Hospital, Wilrijkstraat 10, 2650 Antwerp, Belgium. tom.moreels@uza.be
Telephone: +32-3-8213323 Fax: +32-3-8214478
Received: October 14, 2009 Revised: November 12, 2009 Accepted: November 19, 2009 Published online: February 14, 2010
Abstract
AIM: To investigate the therapeutic effect of Schistosoma mansoni (S. mansoni) soluble worm proteins on gastrointestinal motility disturbances during experimental colitis in mice.
METHODS: Colitis was induced by intrarectal injection of trinitrobenzene sulphate (TNBS) and 6 h later, mice were treated ip with S. mansoni proteins. Experiments were performed 5 d after TNBS injection. Inflammation was quantified using validated inflammation parameters. Gastric emptying and geometric center were measured to assess in vivo gastrointestinal motility. Peristaltic activity of distal colonic segments was studied in vitro using a modified Trendelenburg set-up. Cytokine profiles of T-lymphocytes isolated from the colon were determined by real time reverse transcriptase-polymerase chain reaction.
RESULTS: Intracolonic injection of TNBS caused severe colitis. Treatment with S. mansoni proteins significantly ameliorated colonic inflammation after 5 d. TNBS did not affect gastric emptying but significantly decreased the geometric center and impaired colonic peristaltic activity 5 d after the induction of colitis. Treatment with S. mansoni proteins ameliorated these in vivo and in vitro motility disturbances. In addition, TNBS injection caused a downregulation of effector T cell cytokines after 5 d, whereas a S. mansoni protein effect was no longer observed at this time point.
CONCLUSION: Treatment with S. mansoni proteins attenuated intestinal inflammation and ameliorated motility disturbances during murine experimental colitis.
