Brief Article
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World J Gastroenterol. Mar 14, 2010; 16(10): 1279-1284
Published online Mar 14, 2010. doi: 10.3748/wjg.v16.i10.1279
Influence of efflux pump inhibitors on the multidrug resistance of Helicobacter pylori
Zhan Zhang, Zhi-Qiang Liu, Peng-Yuan Zheng, Fu-Ai Tang, Ping-Chang Yang
Zhan Zhang, Zhi-Qiang Liu, Peng-Yuan Zheng, Fu-Ai Tang, Department of Gastroenterology, the Second Affiliated Hospital of Zhengzhou University, Zhengzhou 450014, Henan Province, China
Ping-Chang Yang, Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario, L8N4A6, Canada
Author contributions: Zhang Z, Liu ZQ, Zheng PY contributed equally to this work; Zheng PY, Tang FA, Liu ZQ and Yang PC designed the research; Zhang Z, Liu ZQ performed the research; Zheng PY contributed to the new reagents/analytic tools; Zhang Z analyzed the data; Zhang Z, Liu ZQ wrote the paper.
Supported by Henan Distinguished Junior Scholar Grant, No. 074100510017
Correspondence to: Dr. Peng-Yuan Zheng, Department of Gastroenterology, the Second Affiliated Hospital of Zhengzhou University, 2 Jingba Road, Zhengzhou 450014, Henan Province, China. medp7123@126.com
Telephone: +86-371-65261035 Fax: +86-371-63934118
Received: December 8, 2009
Revised: January 16, 2010
Accepted: January 23, 2010
Published online: March 14, 2010
Abstract

AIM: To evaluate the effect of efflux pump inhibitors (EPIs) on multidrug resistance of Helicobacter pylori (H. pylori).

METHODS: H. pylori strains were isolated and cultured on Brucella agar plates with 10% sheep’s blood. The multidrug resistant (MDR) H. pylori were obtained with the inducer chloramphenicol by repeated doubling of the concentration until no colony was seen, then the susceptibilities of the MDR strains and their parents to 9 antibiotics were assessed with agar dilution tests. The present study included periods before and after the advent of the EPIs, carbonyl cyanide m-chlorophenyl hydrazone (CCCP), reserpine and pantoprazole), and the minimum inhibitory concentrations (MICs) were determined accordingly. In the same way, the effects of 5 proton pump inhibitors (PPIs), used in treatment of H. pylori infection, on MICs of antibiotics were evaluated.

RESULTS: Four strains of MDR H. pylori were induced successfully, and the antibiotic susceptibilities of MDR strains were partly restored by CCCP and pantoprazole, but there was little effect of reserpine. Rabeprazole was the most effective of the 5 PPIs which could decrease the MICs of antibiotics for MDR H. pylori significantly.

CONCLUSION: In vitro, some EPIs can strengthen the activities of different antibiotics which are the putative substrates of the efflux pump system in H. pylori.

Keywords: Multidrug efflux pump; Helicobacter pylori; Multidrug resistance; Proton pump inhibitor; Real-time polymerase chain reaction