Kim BJ, Park KJ, Kim HW, Choi S, Jun JY, Chang IY, Jeon JH, So I, Kim SJ. Identification of TRPM7 channels in human intestinal interstitial cells of Cajal. World J Gastroenterol 2009; 15(46): 5799-5804 [PMID: 19998500 DOI: 10.3748/wjg.15.5799]
Corresponding Author of This Article
Seon Jeong Kim, PhD, Center for Bio-Artificial Muscle and Department of Biomedical Engineering, Hanyang University, 17 Haengdang-Dong, Seongdong-gu, Seoul 133-791, South Korea. sjk@hanyang.ac.kr
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Original Article
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World J Gastroenterol. Dec 14, 2009; 15(46): 5799-5804 Published online Dec 14, 2009. doi: 10.3748/wjg.15.5799
Identification of TRPM7 channels in human intestinal interstitial cells of Cajal
Byung Joo Kim, Kyu Joo Park, Hyung Woo Kim, Seok Choi, Jae Yeoul Jun, In Youb Chang, Ju-Hong Jeon, Insuk So, Seon Jeong Kim
Byung Joo Kim, Hyung Woo Kim, School of Korean Medicine, Pusan National University, Yangsan 626-870, South Korea
Kyu Joo Park, Department of Surgery, College of Medicine, Seoul National University, Seoul 110-799, South Korea
Seok Choi, Jae Yeoul Jun, Department of Physiology, College of Medicine, Chosun University, Gwangju 501-759, South Korea
In Youb Chang, Department of Anatomy, College of Medicine, Chosun University, Gwangju 501-759, South Korea
Ju-Hong Jeon, Insuk So, Center for Bio-Artificial Muscle and Department of Physiology, College of Medicine, Seoul National University, Seoul 110-799, South Korea
Seon Jeong Kim, Center for Bio-Artificial Muscle and Department of Biomedical Engineering, Hanyang University, Seoul 133-791, South Korea
Author contributions: Kim BJ, Park KJ, Kim HW, Choi S, and Kim SJ designed the research; Kim BJ, Park KJ, Jun JY, and Chang IY performed the research; Jeon JH and So I contributed new reagents and analytic tools; Kim BJ, So I and Kim SJ analyzed the data; and Kim BJ, So I, and Kim SJ wrote the paper.
Supported by The Creative Research Initiative Center for Bio-Artificial Muscle of the Ministry of Education, Science and Technology (MEST) in Korea
Correspondence to: Seon Jeong Kim, PhD, Center for Bio-Artificial Muscle and Department of Biomedical Engineering, Hanyang University, 17 Haengdang-Dong, Seongdong-gu, Seoul 133-791, South Korea. sjk@hanyang.ac.kr
Telephone: +82-2-22202321 Fax: +82-2-22912320
Received: August 6, 2009 Revised: September 24, 2009 Accepted: November 1, 2009 Published online: December 14, 2009
Abstract
AIM: To investigate the characteristics of slow electrical waves and the presence of transient receptor potential melastatin-type 7 (TRPM7) in the human gastrointestinal (GI) tract.
METHODS: Conventional microelectrode techniques were used to record intracellular electrical responses from human GI smooth muscle tissue. Immunohistochemistry was used to identify TRPM7 channels in interstitial cells of Cajal (ICCs).
RESULTS: The human GI tract generated slow electrical waves and had ICCs which functioned as pacemaker cells. Flufenamic acid, a nonselective cation channel blocker, and 2-APB (2-aminoethoxydiphenyl borate) and La3+, TRPM7 channel blockers, inhibited the slow waves. Also, TRPM7 channels were expressed in ICCs in human tissue.
CONCLUSION: These results suggest that the human GI tract generates slow waves and that TRPM7 channels expressed in the ICCs may be involved in the generation of the slow waves.
Kim BJ, Park KJ, Kim HW, Choi S, Jun JY, Chang IY, Jeon JH, So I, Kim SJ. Identification of TRPM7 channels in human intestinal interstitial cells of Cajal. World J Gastroenterol 2009; 15(46): 5799-5804 [PMID: 19998500 DOI: 10.3748/wjg.15.5799]
