Pan GQ, Ren HZ, Zhang SF, Wang XM, Wen JF. Expression of semaphorin 5A and its receptor plexin B3 contributes to invasion and metastasis of gastric carcinoma. World J Gastroenterol 2009; 15(22): 2800-2804 [PMID: 19522033 DOI: 10.3748/wjg.15.2800]
Corresponding Author of This Article
Ji-Fang Wen, Professor, Department of Pathology, Xiangya Medical College, Central South University, Changsha 410008, Hunan Province, China. jifangwen@hotmail.com
Article-Type of This Article
Brief Articles
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Guo-Qing Pan, Hong-Zheng Ren, Ji-Fang Wen, Department of Pathology, Xiangya Medical College, Central South University, Changsha 410008, Hunan Province, China
Guo-Qing Pan, Department of Pathology, the first Affiliated Hospital of Kunming Medical College, Kunming 530032, Yunnan Province, China
Shu-Fang Zhang, Central Laboratory, Affiliated Haikou Hospital of Xiangya Medical College, Central South University, Haikou 570208, Hainan Province, China
Xi-Mei Wang, Department of Basic Medicine, Huaihua Medical College, Huaihua 418000, Hunan Province, China
Author contributions: Pan GQ and Ren HZ contributed equally to this work; Pan GQ designed the research and wrote the manuscript; Ren HZ analyzed the data by Western blotting and RT-PCR; Zhang SF dealt with the statistical data; Wang XM collected the samples; Wen JF revised the manuscript.
Correspondence to: Ji-Fang Wen, Professor, Department of Pathology, Xiangya Medical College, Central South University, Changsha 410008, Hunan Province, China. jifangwen@hotmail.com
Telephone: +86-731-2650400
Fax: +86-731-2650400
Received: October 23, 2008 Revised: May 5, 2009 Accepted: May 12, 2009 Published online: June 14, 2009
Abstract
AIM: To investigate the protein and mRNA expression of semaphorin 5A and its receptor plexin B3 in gastric carcinoma and explore its role in the invasion and metastasis of gastric carcinoma.
METHODS: Expression of semaphorin 5A and its receptor plexin B3 in 48 samples of primary gastric carcinoma, its corresponding non-neoplastic mucosa, and matched regional lymph node metastasis was assayed by reverse transcription-polymerase chain reaction (RT-PCR), real-time RT-PCR and Western blotting.
RESULTS: The protein and mRNA expression of semaphorin 5A and its receptor plexin B3 increased gradually in non-neoplastic mucosa, primary gastric carcinoma and lymph node metastasis (P < 0.05). Moreover, the expression of semaphorin 5A was closely correlated with that of plexin B3.
CONCLUSION: Semaphorin 5A and its receptor plexin B3 play an important role in the invasion and metastasis of gastric carcinoma.
